Detection of polymorphism in Kiss1 and GDF9 gene and its association with growth and reproduction traits in indigenous goats

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dc.contributor.advisorMagotra, Ankit
dc.contributor.authorVikram Jeet
dc.date.accessioned2020-02-06T09:31:59Z
dc.date.available2020-02-06T09:31:59Z
dc.date.issued2019
dc.description.abstractThe present study was conducted on randomly selected 84Beetal goat, 47 Jakhrana goat and 62 indigenous goat breeds(from farmers flock) to scan the polymorphism in Kiss1 and GDF9 gene and its association with growth and reproduction traits. Two sets of primers were used to amplify targeted regions of Kiss1 and GDF9 gene in Beetal, Jakhrana and field goat samples. The PCR product of 242 bp size of targeted region of Kiss1 gene was digested with the restriction enzyme Sac1. All the three possible genotypes were observed in our resource population. Frequency of C allele of Kiss1 gene was higher in our studied population goats i.e. 0.89 in Beetal, 0.86 in Jakhrana and 0.73 in field samples. CC genotype was found to be the predominant genotype Beetal (0.81), Jakhrana (0.72) and goats at farmers flock (0.51). The 1069 bp PCR product of GDF9 gene was digested with the DdeI enzyme, resolved into monomorphic pattern AA in field samples but three polymorphic patterns of AA, AB and BB types in Beetal goat and two genotype in Jakhrana goat. The genotypic and allelic frequencies of GDF9 gene indicated that the frequency of A allele was highest in Beetal (0.86), Jakhrana (0.98) and goats at farmers flock (1.00). Effect of Kiss 1 genotype was non-significant in all growth and reproductive traits except 12M body weight in Beetal goats. CC genotype was having significant higher (P<0.05) 12M body weight (22.57) than CT genotype (19.64). In field samples, CT genotype had significant association with litter size. CT and TT genotypes had significantly higher litter size (P<0.01) than CC genotype indicating that allele T was associated with higher litter size. Effect of GDF 9 genotype was non-significant in all growth and reproductive traits except litter size in Beetal goats. AB genotyped Beetal goats showed significantly (P<0.05) highest average litter size 1.592±0.07 compared to AA genotype 1.139±0.05 indicating that allele B was associated with higher litter size. Breed-wise distribution of field samples revealed that Barbari goats showed significantly higher average litter size (2.857±0.11) compared to Beetal, Non-descript, Sirohi and Sojat goat breeds under study (P<0.01). The frequency of CT and TT genotype is also comparatively higher in Barbari goats. In Beetal goat, combined genotypes i.e ABCC (1.67±0.086) and ABCT (1.45±0.141) significantly revealed highest average litter size compared to other genotypes. (P<0.01).These observations on SNPs of Kiss1 and GDF9 gene and their association with growth and reproduction traits can be utilized as a marker after validation of results on large population.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810142626
dc.keywordsBeetal, Jakhrana, litter size, Kiss1, GDF9en_US
dc.language.isoenen_US
dc.publisherLUVASen_US
dc.subAnimal Genetics and Breedingen_US
dc.subjectnullen_US
dc.themeDetection of polymorphism in Kiss1 and GDF9 gene and its association with growth and reproduction traits in indigenous goatsen_US
dc.these.typeM.V.Sc.en_US
dc.titleDetection of polymorphism in Kiss1 and GDF9 gene and its association with growth and reproduction traits in indigenous goatsen_US
dc.typeThesisen_US
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