FORMULATION, OPTIMIZATION AND EVALUATION OF ENROFLOXACIN SOLID LIPID NANOPARTICLES FOR SUSTAINED ORAL DELIVERY
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Date
2015
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Abstract
Objective: The objective of this study was to formulate and evaluate enrofloxacin solid lipid nanoparticles (SLNs) using a hot homogenization coupled
with ultrasonication method for sustained oral delivery.
Methods: The SLNs were prepared using tripalmitin as lipid carrier, tween 80 and span 80 as surfactants and polyvinyl alcohol (PVA) as a stabilizer.
The factors such as composition and concentration of lipid carrier and surfactant on the particle size were investigated to optimize the formulations.
The optimized SLNs formulations were utilized to entrap enrofloxacin and characterized for particle size, polydispersity index (PDI), zeta potential
(using dynamic light scattering), shape (using atomic force microscopy (AFM) and transmission electron microscopy [TEM]), drug encapsulation
efficiency (EE) , loading capacity (LC) (using by dialysis and ultracentrifugation methods), and in vitro drug release (using by dialysis). The prepared
SLNs were analyzed by Fourier transform infrared (FT-IR) spectroscopy to confirm the cross-linking reaction between drug, lipid and surfactants.
Results: The results demonstrated that the particle size, PDI, zeta potential, EE and LC of the enrofloxacin SLNs were 154.72±6.11 nm, 0.42±0.11,
−28.83±0.60 mV, 59.66±3.22% and 6.13±0.32%, respectively. TEM and AFM images showed spherical to circular particles with well-defined periphery.
In vitro drug release exhibited biphasic pattern with an initial burst release of 18% within 2 hrs, followed by sustained release over 96 hrs. FT-IR
study suggested that during the process of formulations, lipid and surfactants have not reacted with the drug to give rise to reactant products and it
was only physical mixture.
Conclusion: The results indicated that SLNs might be a promising delivery system to prolong and enhance the pharmacological activity of enrofloxacin.
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Veterinary Science, Veterinary Pharmacology and Toxicology