Comparative studies on urinary metabotypes and scrotal thermal signatures in normal and cloned Murrah buffalo bulls
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Date
2022-10
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Lala lajpat rai university Hisar
Abstract
The present study conducted on fourteen Murrah buffalo bulls - ten growing (five each, cloned and
normal) and four adult (two each, cloned and normal), evaluated urinary metabolites, skin surface
temperatures of different body parts (with particular focus on scrotum) and hemato-biochemical
parameters in scrotal thermal signatures in normal and cloned buffalo bulls across four seasons
(summer, rainy, autumn and winter) over one year. There was no insignificant difference in body
surface temperature of normal and cloned animals except winter season. The scrotal skin surface
temperature was significantly high in cloned animals as compare to normal growing (p<0.05) animals.
Among different body parts recorded for surface temperatures, the eye temperature had highest
correlation with rectal temperature and was also found closed to it at any point of time, suggesting its
use as an alternate, non – touch method for recording internal body temperatures. The growth rate and
blood-biochemical parameters recorded insignificant differences between normal and cloned animals.
1H-NMR spectroscopy suggested that there are qualitative and quantitative differences in the identified
136-140 urine metabolites, between cloned and normal animals in both groups (i.e. adult and growing).
The differences were marked in summer and winter seasons in adult cloned animals. Of the 25 most
abundant metabolites identified nine viz. L-arabinitol, xylose, glucose-1-phosphate, uridine diphosphate glucose (UDP-glucose), L-isoleucine, L-leucine, methylmalonic acid, keto-leucine and L valine were found in higher concentration in urine of cloned animals. Contrarily, four metabolites of
these abundant metabolites viz. myo-inositol, glucuronic acid, taurine and glycine metabolite showed
high relative abundance in the normal animals. The functional bioinformatics suggest significant
differences in urinary metabolites between cloned and normal animals w.r.t. thermoregulation, energy
metabolism, hormone synthesis and degenerative changes. Study also points towards higher scrotal
temperature in growing cloned animals. The thermal screening conducted in the study also suggest that
ocular temperature can serve as an alternate, non-touch method for estimating internal body
temperature in buffaloes