Morpho-molecular characterization of three line Rice Hybrids
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Date
2022
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DRPCAU, PUSA
Abstract
The current investigation on “Morpho- molecular characterization of threeline
rice hybrids” was carried out with 31 three-line rice hybrids and 3 commercial
checks. Objectives of the experiment were to assess the agro-morphological attributes
and production potential of three-line rice hybrids, molecular marker-based
characterization of promising three lines rice hybrid.
The experiment was conducted Hybrid Rice plot of TCA, Dholi, Muzaffarpur
and Molecular investigation was done at Molecular laboratory, Post graduate
Department of Genetics & Plant Breeding, RPCAU, Pusa, Bihar, India. The
experimental design adopted was Randomized Complete Block Design (RBD) with
three replications. The observation for 18 quantitative attributes i.e. plant height, days
to 50% flowering, number of tillers per plant, number of panicles per plant, panicle
length, leaf length, leaf width, leaf area, culm diameter, kernel length, kernel width,
root volume, root fresh weight, root dry weight, days to maturity, spikelet fertility, test
weight and grain yield per plant were recorded for estimation of various parameters
like genetic variability, phenotypic correlation coefficient, path analysis and
estimation of standard heterosis for yield. Molecular characterization was undertaken
for estimation of diversity and as confirmation test of F1 hybrid genotypes with their
respective parental lines. The results of the analysis of variance showed that all
character genotypes differed significantly.
The results of the analysis of variance showed that all character genotypes
differed significantly.
Among the checks the variety the Rajendra Sweta performed best for the grain
yield per plant. Two rice hybrid genotypes namely IR68897A × KMR-3R and
Rajendra-3A × RRR–4 was exhibited superior standard heterosis over all three checks
for trait grain yield per plant. Five rice hybrid genotypes namely Rajendra-1A ×
RRR–4, Rajendra-3A × DR714 -1-2, Rajendra-3A × MSN- 36 R, IR - 58025A ×
MSN - 36R and CMR 32A × RRR – 2 also exhibited superior standard heterosis for
yield over two checks Rajendra Bhagwati and Arize gold 6444.
Plant height, number of tillers per plant, number of panicles per plant, leaf
length, leaf area, kernel length, kernel width, root fresh weight, root dry weight,
spikelet fertility, test weight, showed positive significant correlation with grain yield
per plant. Hence, selection for any one of these characters would ultimately bring
improvement of grain yield.
No. of tillers per plant, plant height, root fresh weight, leaf length, no. of
panicles per plant, leaf width, kernel length, root volume revealed a significant direct
impact on grain yield per plant. Therefore, making a selection based on these traits
would increase grain output.
In terms of standard heterosis for yield, IR68897A×KMR-3R, Rajendra-
3A×RRR-4 are shown positively superior for yield over three checks followed by
Rajendra - 1A × RRR – 4, R Rajendra - 3A × DR714 - 1- 2, Rajendra - 3A × MSN-
36 R, IR - 58025A × MSN - 36R, CMR 32A × RRR – 2 have shown positively
superior for yield over two checks Rajendra Bhagwati and Arize gold 6444,
remaining hybrids sown superiority over one check for yield.
By utilising 12 primer pairs, a total of 33 shared alleles and 13 unique alleles
were produced as amplified products.
Allele diversity and frequency among the rice parental genotypes ranged from
0.00 in RM 416, RM 431 and RM 558 to 0.84 on MRG 2894, according to the PIC
values.
The range of alleles per locus was between one in primer pair RM-416, RM-
431, RM-558 to seven in primer pair MRG-2894 and six in primer pair RM-520, RM-
538, RM-515 & five in primer pair RM-555, four in primer pair RM-276 and three in
primer pair RM-252, RM-319, RM-321.
All primers pairs were generated both unique and shared alleles except RM-
319, RM-416, RM-431 and RM-558 are generated only shared alleles without any
unique alleles.
The magnitude dice similarity coefficient between RAJ-3A and IR 68897A
was found to be maximum (0.917) whereas it was minimum for IR 58025A and RRR-
1, RRR-5, DR-714-1-2 (0.240).
The dendrogram makes it quite evident that the entries were essentially split
into five clusters. Cluster B had maximum (4) genotypes followed by cluster C, D and
E (2) genotypes, cluster A is mono-genotypic.
Among 12 primers seven primers found to be comparatively informative for
all nineteen hybrids and eleven parents. Only five primers namely MRG2894,
RM515, RM 520, RM 538 and RM 555 were able to confirm the F1 with respective
parental lines.