“Effect of Growth Regulators on in vitro regeneration and germination behavior of Buchanania cochinchinensis (Lour.) Almeida”
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Date
2023-09-15
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Forest Biology and Tree Improvement Banda University of Agriculture and Technology Banda (U.P)
Abstract
Buchanania cochinchinensis (Lour.) M.R. Almeida is commonly known as chironji belonging to
the family Anacardiaceae. It is an excellent economic fruit tree of agro-forestry and social
forestry. The species is highly heterozygous and cross pollinated with gestation period of 15-20
years. It has multifarious uses as seed are edible and of high medicinal value. It attains the height
up to 20 m and distributed in the states of Madhya Pradesh, Chhattisgarh, Jharkhand, Gujarat,
Uttar Pradesh, Maharashtra, Orissa, Andhra Pradesh. The species is listed as a vulnerable
medicinal plant in the International Union for Conservation of Nature and Natural Resources
(IUCN) Red Data Book. Due to heavy unscientific extraction and lack of proper propagation
protocol, there is a tremendous pressure on the natural genetic resources of the species. In view of
the facts, the present study was carried out to investigate effect of various growth regulators on
germination behavior of chironji seed and in vitro response of the chironji explants. The results
revealed that BA 600ppm was proven best among all treatments with maximum values of
germination percentage (73.67%), germination speed/rate (0.69), MDG (2.50), PV (15.00) & GV
(37.50). In vitro response revealed that maximum value (24.67%) of callusing was found in
internodal explants with minimum days of response of 12 days only in MS media supplemented
with 2,4-D + NAA (0.9+0.6) whereas GA3 (2.0 mg/l), which was able to induce organogenesis in
the cultures established through zygotic embryo with only 2% of morphogenic response in 27
days. The present study on germination behavior and in vitro studies will help the scientific
community and other stakeholders to adopt seed pre-sowing treatment and in vitro approaches for
the production of quality planting material of chironji for afforestation and reforestation purposes
with high survival percentage on nursery and field plantation.
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Citation
Amarjeet Thakur {2023} Effect of Growth Regulators on in vitro regeneration and germination behavior of Buchanania cochinchinensis (Lour.) Almeida”, M.Sc. (Horti.), Forest Biology and Tree Improvement Banda University of Agriculture and Technology Banda (U.P)