Identification and expression profiling of Banana bract mosaic virus (BBrMV) responsive microRNAs in banana cultivar nendran (Musa AAB)

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dc.contributor.advisorSoni, K B
dc.contributor.authorAthira, Vijayan
dc.contributor.authorKAU
dc.date.accessioned2021-09-06T07:11:08Z
dc.date.available2021-09-06T07:11:08Z
dc.date.issued2020
dc.descriptionPGen_US
dc.description.abstractThe study entitled “Identification and expression profiling of Banana bract mosaic virus (BBrMV) responsive microRNAs in banana cultivar Nendran (Musa AAB)” was conducted in the Department of Plant Biotechnology, College of Agriculture Vellayani, Thiruvananthapuram during 2018-2020. The objective of the study was to validate computationally predicted selected microRNAs (miRNAs) in banana cultivar Nendran (Musa AAB) and to study their expression in Banana bract mosaic virus (BBrMV) infection. In a previous study conducted in the Department of Plant Biotechnology, College of Agriculture Vellayani, 52 mature miRNAs were computationally predicted in the banana genome using NOVOMIR tool and their target genes identified. From them, three miRNAs were selected to study their response to BBrMV infection. The selection was done based on the possible role of their target genes in biotic stress responses. The miRNAs selected were miR-5417 (target: F Box family protein), miR- 1634 (target: Patatin like phospholipase family protein) and miR- 67 (target: Probable xyloglucan endotransglucosylase/hydrolase protein 33). The study was carried out in the BBrMV infected field grown banana plants of cv. Nendran. BBrMV infection in the infected plants were confirmed by RT-PCR using viral coat protein gene specific primers and the expected amplicon of size 745 bp was obtained. Uninfected plants served as control. For studying the expression of these miRNAs, RNA was isolated from the leaves of infected and control plants samples following the modified Rodrigues-Garcia protocol. Stem-loop primers were used for reverse transcribing miRNAs to cDNA. The primers for the amplification of miRNAs and their target genes were designed, synthesized and their specificity was analyzed. The expression profiling of miRNAs and their target genes was done by RT-qPCR and the results were analyzed using qBase plus software. The Raw Cq values obtained were normalized using a reference gene β-actin. The miRNAs and their target genes were analysed with reference to the uninfected control plants. RT-qPCR analysis confirmed the presence of all the three computationally predicted miRNAs in banana cv Nendran. All the BBrMV infected samples showed an elevated level of miR-1634 (2.95 and 6.52 fold) and miR-67 (4.46 and 5.04 fold) compared to the uninfected control. Similarly, their target genes Patatin like phospholipase family protein (target of miR-1634) showed 5 to 10 fold increase and Probable xyloglucan endotransglucosylase/hydrolase protein 33 (target of miR- 67) showed 6.95 and 9.45 fold increase in the infected plants. No considerable change in the expression of miR-5417 was observed in the infected samples. But its target gene (F Box family protein) was found upregulated by 6.3 to 9.2 folds in both BBrMV infected plants compared to the control. Hence an inverse correlation could be established between the miRNA and its target. The study showed that all the three miRNAs (miR-5417, miR-67 and miR-1634) respond to BBrMV infection and have a role in stress response of the plants. All the target genes showed upregulation in the BBrMV infected samples, showing their role in biotic stress response. miR-5417 showed an inverse correlation with its target.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810175086
dc.keywordsbanana cultivar Nendran, Banana bract mosaic virusen_US
dc.language.isoEnglishen_US
dc.pages78p.en_US
dc.publisherDepartment of Plant Biotechnology, College of Agriculture, Vellayanien_US
dc.subPlant Biotechnologyen_US
dc.themeIdentification of Banana bract mosaic virusen_US
dc.these.typeM.Scen_US
dc.titleIdentification and expression profiling of Banana bract mosaic virus (BBrMV) responsive microRNAs in banana cultivar nendran (Musa AAB)en_US
dc.typeThesisen_US
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