STUDIES ON BIO-PRIMING OF PADDY SEEDS TO COMBAT BLAST DISEASE (Magnaporthe oryzae L.)
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Date
2016-08-20
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
A study was conducted in the laboratory, greenhouse and field to know the
influence of bio-priming on paddy seeds against blast disease at GKVK, Bengaluru and
VC, Farm Mandya. The total 60 bacterial strains were isolated from paddy rhizosphere
and used for in vitro and in vivo evaluation against M. oryzae along with two reference
strains B. subtilis and P. fluorescens and eleven bacterial strains showed maximum
inhibition. Thirteen strains were characterized as bacteria belong to genera Bacillus,
pseudomonas, Serratia, Alcaligenes and Proteus using 16S rRNA sequencing. The
bacterial isolates exhibited antagonist property showed the presence of antimicrobial
peptide genes. In vitro evaluation revealed that the B. amyloliquefaciens UASBR9 and
Serratia marcescens UASBR4 found most effective against the pathogen (84.14 and
76.83 per cent respectively) compared to reference cultures RBs-1 (72.00 %) and RPf-1
(55.24 %). The least PDI and highest germination was recorded in HR-12 seeds bioprimed
with UASBR9 (0.69 and 99.00 %) compared to untreated control (3.43 and 95.00
%) under in vivo condition. Seeds bio-primed with formulated product of UASBR9 and
talc+MgSO4 @10g/kg seed was recorded least PDI (6.63 %), highest seed yield/plot (0.68
kg) and maximum germination (99.50 %) compared to untreated control (20.25 %, 0.27
kg and 90.00 %, respectively). Seed bio-primed with UASBR9 showed induced systemic
resistance in rice on challenge inoculation with M. oryzae and increased activities of
peroxidase (0.563 min-1 gram-1), PPO (0.825 min-1 gram-1) and PAL (6.849 nmol t-CA)
and defense-related enzymes response against pathogen compared to untreated control.
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