Gene scouting for rust(s) resistance in wheat germplasm

dc.contributor.advisorJaspal Kaur
dc.contributor.authorSandeep Singh
dc.date.accessioned2018-11-29T14:49:05Z
dc.date.available2018-11-29T14:49:05Z
dc.date.issued2018
dc.description.abstractDuring the Rabi crop season of the year 2016-17, six hundred and seven (607) wheat germplasm lines were evaluated in the field and found sixty six lines resistant to stripe rust, three hundred thirty six lines resistant to leaf rust and thirty four lines resistant to both stripe rust and leaf rust. In the second year (2017-18), the wheat germplasm was evaluated against different pathotypes of Puccinia striiformis tritici (Pst) (238S119, 110S119, 46S119 and 78S84) and P. triticina (Pt) (77-5 and 77-9) at seedling stage. From seedling reaction test it was observed that fifty six (56) lines and one hundred and ten lines (110) possess resistance against all the pathotypes of Pst and Pt tested respectively. All the test wheat lines were categorized into different clusters based on final rust severity (FRS), area under disease progress curve (AUDPC), relative area under disease progress curve (rAUDPC) and coefficient of infection (CI). Fifty two (52) lines were highly resistant to stripe rust and one hundred seventy one (171) lines were highly resistant to leaf rust and remaining lines showed different reaction based on the particular range of slow rusting parameters. Genotypes which showed resistance either to stripe rust or to leaf rust were further evaluated against mixture of Pst pathotypes (238S119, 110S119, 46S119 and 78S84) and individually against 110S119 and 46S119. For leaf rust evaluation was done by using mixture of Pt pathotypes (77-5, 77-9, 104-2 and 12-2) and 77-5 and 77-9 in isolation. NILs carrying known APR genes and susceptible checks for also used for comparison purpose under the field conditions. The results of the present study revealed that fifty two lines has resistance against most prevalent pathotypes of stripe rust in Punjab (238S119, 110S119, 46S119 and 78S84) and one hundred seventy one (171) lines have resistance to mixture (77-5, 77-9, 104-2 and 12-2) pathotypes of Pt. Reaction on NILs showed that Yr1, Yr5, Yr10, Yr15, Yr24, Yr26, Yrsp, Yr31, Yr51, Yr47, Yr57, Yr63 are effective against most prevalent pathotypes of stripe rust in Punjab and Lr2a, Lr2b, Lr8, Lr19, Lr22a, Lr23, Lr(27+31), Lr45, Lr51, Lr53, Lr57, Lr58, Lr76 are effective against leaf rust pathotypes. For deployment of resistance sources, total 40 crosses were made. Eleven lines resistant to stripe rust were crossed with PBW621 and twenty nine lines resistant to leaf rust were crossed with HD3086. F1 and F2 plants obtained were tested separately against stripe rust and leaf rust. From the disease reaction of majority of F1 and F2 populations it was concluded that resistance in these lines is controlled by single dominant gene against both stripe and leaf rust.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810084832
dc.keywordsGermplasm, Leaf rust, Stripe rust, Resistance, wheaten_US
dc.language.isoenen_US
dc.pages88en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemGene scouting for rust(s) resistance in wheat germplasmen_US
dc.subPlant Pathologyen_US
dc.subjectnullen_US
dc.themeGene scouting for rust(s) resistance in wheat germplasmen_US
dc.these.typeM.Scen_US
dc.titleGene scouting for rust(s) resistance in wheat germplasmen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
M.Sc. Thesis.pdf
Size:
2.03 MB
Format:
Adobe Portable Document Format
Description:
M.Sc.
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Collections