In vitro shoot tip grafting studies in Kinnow mandarin

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Date
2012
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Publisher
CCSHAU
Abstract
In the present investigation, four citrus rootstocks, viz. Sour orange, Carrizo citrange, Rough lemon and Cleopatra mandarin were used to study their in vitro seed germination and the effect of rootstocks and age of seedlings on success of in vitro shoot tip grafting in Kinnow mandarin. The surface sterilization treatment of seeds of rootstocks with ethanol (70%) for 30 seconds followed by HgCl2 (0.1%) for 5 minutes was found better. Quickest and highest seed germination was recorded in Rough lemon followed by Cleopatra mandarin. Highest number of seedlings per seed was recorded in Carrizo citrange. Shoot length and root length of the seedlings was recorded highest in Rough lemon. The maximum STG success was observed in Carrizo citrange on 12 days old seedlings. STG on Rough lemon rootstock took minimum number of days to bud sprout followed by Carrizo citrange. The highest number of leaves per graft was recorded on 12 day old seedlings of Carrizo citarange followed by on Rough lemon. The highest scion shoot length was observed on 12 day old seedlings of Carrizo citrange followed by that of 16 day old seedlings of Rough lemon. The maximum number of roots per graft was produced in Carrizo citrange. The Highest per cent transplanting success of STG plants was observed on Carrizo citrange followed by Rough lemon. It was observed that the Carrizo citrange found best for most of STG parameter, viz. per cent STG success, number of leaves per graft, scion shoot length, number of roots per graft, stock root length and transplanting success.
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Keywords
Citrus rootstocks, Shoot tip gng, Stock, Scion, Kinnow mandarin, Transplanting success
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