CLONING OF ENDOCHITINASE AND ENDOGLUCANASE GENES FROM NATIVE ISOLATES Trichoderma
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Date
2009-06
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University of Agricultural Sciences Dharwad
Abstract
Eighty six Trichoderma isolates previously isolated and maintained in the Department of Biotechnology, were screened against Sclerotium rolfsii, Rhizoctonia solani and Colletotrichum capsici through dual plate assay. Based on growth inhibition, they were grouped into efficient, moderate and poor isolates. Further, endochtinase (ech42) and endoglucanase (, 1-6 endoglucanase) genes were cloned from efficient, moderate and poor isolates of T. harzianum using specific primers. Differences were not observed in the amino acid sequences of ech42 cloned from efficient, moderate and poor isolates of same species (T. harzianum). However, ech42 cloned from most efficient isolates of T. virens showed differences at amino acid level in 15 positions compared to ech42 cloned from T. harzianum. Further, the , 1-6 glucanase gene cloned from efficient, moderate and poor isolates showed differences at amino acid level. The gene cloned from efficient (IABT 1041) T. harzianum isolates differed from other two cloned from moderate and poor isolates (IABT 1046 and IABT 1054) in 10 amino acid positions and the , 1-6 glucanase cloned from IABT 1046 and IABT 1054 differed at 2 amino acid positions. The cloned genes can be further subjected for expression and bioassay studies to know their utility in development of transgenic plants.
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