Effect of seed treatment with proline and salicylic acid on germination in tinda (Citrullus vulgaris L.) cv. S-48 at different temperatures
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Date
2016
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Punjab Agricultural University, Ludhiana
Abstract
The effect of seed treatments with proline and salicylic acid on some physiological and
biochemical parameters of tinda (Citrullus vulgaris L.) cv.S-48 seeds were studied. Seeds of
tinda were treated with different concentrations of proline (1mM, 5mM, 10mM and 20mM)
and salicylic acid (0.1mM, 0.5mM, 1mM and 5mM) for 5 hours at 25°C and 20°C. After 12
days of germination, seedlings were collected for physiological and biochemical examination.
All the seed treatments increased the physiological parameters such as percent germination,
seedling length, fresh weight, dry weight, vigour index I and II and speed of germination as
compared to control. The treatments decreased the electrolyte leakage as compared to control
and increased biochemical parameter like total soluble sugar, proteins, activity of antioxidant
enzymes like catalase, peroxidase and superoxide dismutase as compared to control at both
the temperatures. Hydration and warm water treatments also increased different physiological
and biochemical parameters. Proline 20mM was more effective than proline (1, 5 and 10
mM). Similarly, salicylic acid 1mM was effective maximally among salicylic acid
concentrations (0.1, 0.5 and 5 mM) at both the temperatures.
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