Microsatellite analysis for targeting bacterial leaf blight resistance in cluster bean [Cyamopsis tetragonoloba (L.) Taub.]
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Date
2018
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CCSHAU
Abstract
Cluster bean [Cyamopsis tetragonoloba (L.) Taub.], a drought tolerant and industrially important forage legume because of galactomannan (also known as guar gum) present in the endosperm of seeds. There is huge demand of guar gum but the production is less than demand. Bacterial leaf blight (BLB) is the major constraint which causes yield losses up to 60-80%. There is a need to develop high yielding and disease resistant varieties. Segregating populations F3-4 and F4-5 along with parents of interspecific cross (HG563 × C. serrata) and intraspecific cross (HG75 × PNB) respectively were evaluated for various morphological traits and bacterial leaf blight resistance under natural condition. A total 192 SSRs comprising EST SSR from cluster bean and SSRs from other legumes were used in molecular analysis. In interspecific cross, 38 SSRs were found polymorphic while 39 showed polymorphism in intraspecific cross. In intraspecific cross, the segregants grouped with PNB while the population of interspecific cross grouped with HG563. Linkage analysis using polymorphic markers in both the crosses revealed that nine markers formed a single linkage group in interspecific cross and only five markers formed a single linkage group in intraspecific cross while other markers remain unlinked. Four minor QTLs were identified in interspecific cross for plant height (qPH1.1), hundred seed weight (qHSW1.1), disease resistance (qPDI1.1) and gum content (qGC1.1) mapped on single linkage group at 39.41, 123.41, 112.41 and 102.41 cM, respectively. A few promising lines based on phenotypic and molecular analysis have been identified which can be further employed in cluster bean breeding program.
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