Distribution and characterization of Burkholderia species causing panicle blight in paddy and its management
| dc.contributor.advisor | Vishunavat, Karuna | |
| dc.contributor.author | Sudha Nandni | |
| dc.date.accessioned | 2019-12-10T09:24:50Z | |
| dc.date.available | 2019-12-10T09:24:50Z | |
| dc.date.issued | 2019-09 | |
| dc.description.abstract | The bacterial panicle blight of rice is an important bacterial disease which was identified for the first time at Pantnagar in the year 2010-2011, at field though, in low incidences and sporadically. Bacterial panicle blight (BPB) caused by Burkholderia glumae, is an emerging disease which has recently come out as a menace in rice-growing areas of northern India. Considering the importance of crop and continuous spread of the disease, present research was initiated to decipher the prevalence and characterization of the Burkholderia sp. in different regions of northern India and its management strategies during the year 2017-2018 and 2018-2019 at GBPUA&T., Pantnagar. The findings observed under the present investigation are summarized as follows. Symptomatic and asymptomatic seeds and infected plant parts collected from different geographical regions of northern India mostly, including states viz. - Uttarakhand, Haryana, Uttar Pradesh, Bihar and Assam exhibited symptoms of BPB on infected leaf, leaf sheath, base of boot leaf and panicles. A total of 28 bacterial isolates were recovered out of which only 21 isolates were found pathogenic on rice under glass house conditions. Colony characters revealed that 6 different coloured colonies viz. - White (glistening), Creamy white (smooth), Creamy (smooth), Creamy yellow (smooth), Pale yellow and (smooth), Shiny yellow (flattened) were present among the isolates. Biochemical studies revealed that the pathogenic isolates were positive for gelatin liquefaction, KOH Solubility and catalase test while negative for starch hydrolysis, oxidase reaction and arginine dihydrolase test. Molecular characterization of the isolates on the basis of their 16S rDNA sequences confirmed that (14) Burkholderia glumae, (5) B. gladioli and (2) B. plantarii were recovered from the infected samples and thus, showed the prevalence of different Burkholderia sp. causing BPB in the regions of northern India. Under the oil immersion lens of compound microscope (100X) through gram staining the bacterial isolates were found to be gram negative absorbing red colour of the counter stain and rod shaped with round ends. The bacterium was found to be seed-borne. Maximum recovery 93% of the bacterium was recorded from the freshly harvested lot which was gradually reduced to 26% after one year of storage under normal laboratory conditions (14-30°C). Twenty- two paddy varieties were screened for BPB resistance out of which early maturing varieties (105-125 days) Pant Dhan-11, Pant Dhan-16 and Pant Dhan-12, mid maturing varieties (120-130 days) Pant Dhan-19, Pant Dhan-10 and Pant Dhan-28 and late maturing varieties (130-140 days) Pant Dhan-18, Pant Basmati-2, Pant Sugandh Dhan-15, Pant Basmati-1 and Pant Sugandh Dhan-25 exhibited moderate resistant reaction while rest others showed moderate susceptible or susceptible reaction towards the pathogen. In vitro screening of antibiotics at five different concentrations against B. glumae exhibited that the 100 ppm concentration of all the antibiotics were most effective. The minimum disease incidence and disease severity was in copper oxychloride and Copper oxychloride + Norfloxacin. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810137257 | |
| dc.keywords | distribution, characterization, Burkholderia, species diversity, panicle, blight, rice, fungal diseases | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 137 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Rice | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.subject | null | en_US |
| dc.theme | Fungal Diseases | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Distribution and characterization of Burkholderia species causing panicle blight in paddy and its management | en_US |
| dc.type | Thesis | en_US |