THERAPEUTIC EFFICACY OF PGF2α AND GnRH IN REPEAT BREEDER BUFFALOES (Bubalus bubalis)
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Date
2020-01
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517502. (A.P.) INDIA
Abstract
The therapeutic efficacy of GnRH on corpus luteum function and embryonic mortality was investigated in 40 non-infectious repeat breeder graded Murrah buffaloes inseminated towards the end of the breeding season. Non-infectious repeat breeder buffaloes (n=30) were synchronised using double PG protocol followed by AI. In the present study 45 repeat breeder Graded Murrah buffaloes were screened to assess the different causes of repeat breeding. The causes of repeat breeding in Graded Murrah buffaloes included genitilia without any palpable abnormalities (non-infectious), subclinical endometritis and tubal blockage recorded in 40, 5, 0 buffaloes and the percentage incidence was 88.88, 11.12 and 0, respectively. White side test and PSP dye test could be effectively used to diagnosis the subclinical endometritis and tubal blockage, respectively. The selected buffaloes were allowed to skip one estrous cycle for careful observation of estrus parameters such as length of estrous cycle (days), duration of estrus (hrs) and intensity of estrus (points) which were recorded as 21.47±0.40 days, 23.50±0.03 hours, 13.42±0.37 points respectively.
After administration of double PGF2α protocol, the estrus response rate (%), mean time of onset of estrus (hrs), ovulation response rate (%) in 30 repeat breeder buffaloes were 90 per cent (27/30), 54±1.70 hrs and 66.66 per cent (18/27), respectively. The efficacy of GnRH was studied in Group I (20μg of GnRH just before AI at observed estrus), Group II (20μg of GnRH just before AI at observed estrus and 5th day of post AI) and Group III (20μg of GnRH just before AI at observed estrus and 15th day post insemination), in terms of conception rates while untreated repeat breeder buffaloes were kept as controls (Group IV, n=10). The serum concentrations of total cholesterol, total protein, glucose, calcium and phosphorus varied significantly (P<0.05) between conceived and non-conceived buffaloes (higher in conceived than that of non- conceived buffaloes) of Group I, II, III and IV. The results from the present study revealed a significant difference (P<0.01) in the serum progesterone profiles between conceived and non-conceived buffaloes among all the treatment groups on day 30 post AI. However there was no significant difference (P>0.01) in serum progesterone profiles in both conceived and non-conceived buffaloes of all the treatment groups at initiation of hormonal treatment and at induced estrus. These results also revealed significantly higher values of (P<0.05) serum progesterone profile on day 30 post AI compared to other days of collection in conceived buffaloes. The overall conception rates (%) of repeat breeding Graded Murrah buffaloes in Group I, II III and control group (Group IV) were recorded to be 33.33 (3/9), 55.55 (5/9), 55.55 (5/9) and 20 (2/10) per cent, respectively. It was further, concluded that GnRH could be given on the day of AI along with day 5 (Group II) and day 15 (Group III) to achieve higher (55.55 and 55.55 %) conception rates when compared with that of administration of GnRH only on the day of AI (Group I-33.33 %) and control group (20.00 %). It was concluded that application of double PGF2α protocol as a fertility management strategy was effective for induction and synchronization of fertile estrus and ovulation in non-infectious repeat breeder buffaloes. It was further concluded that hormone GnRH could be used to enhance the serum progesterone concentrations when administered on day 5 or day 15 after artificial insemination in repeat breeding buffaloes
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