Bioprocessing of cereal bran for production of low-cost liquid consortium biofertilizer
Loading...
Date
2024
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Punjab Agricultural University
Abstract
The present study was carried out to valorize rice and wheat bran for the development of low-cost liquid consortium biofertilizer (already recommended as charcoal based bioformulation by PAU, Ludhiana). Different media formulations (1:10, 2:10, 3:10, 4:10 and 5:10) of rice/wheat bran with distilled water were evaluated for growth of consortium biofertilizer cultures (Azotobacter chroococcum, Bacillus subtilis and Pseudomonas sp.). The viable cell count gradually enhanced with the increasing substrate concentration, maximum being with 5:10 for both rice (RB5) and wheat bran (WB5). Among bran based bioformulations, wheat bran 5:10 (WB5) exhibited highest viable cell population for Azotobacter chroococcum (10.68±0.09 Log10 CFU/ml) and Bacillus subtilis (12.63±0.04 Log10 CFU/ml) whereas for Pseudomonas sp. rice bran recorded maximum viability of 12.71±0.05 Log10 CFU/ml after 72 hours of incubation. As RB5 and WB5 liquid formulations presented appreciable growth of all consortium cultures, they were further optimized for enhancement of shelf life using 5, 10 and 15mM trehalose, 0.05 and 0.1% carboxymethyl cellulose (CMC) and 0.5 and 1% glycerol. Consortium cultures inoculated in standard nutrient broth and charcoal based consortium biofertilizer (recommended by PAU) were also maintained for comparison. Following the peak growth at 72 hours of incubation, a gradual decrease in viable population of consortium biofertilizer cultures was observed in all the liquid bioformulations. The bran based bioformulations (RB5 and WB5) supplemented with 1% glycerol, 0.1% CMC, 10 and 15mM trehalose were noticed to support the shelf life of 150 days for one or more consortium bacterial cultures. The WB5 and RB5 bioformulations with 15mM trehalose and 0.1% CMC, not only recorded significantly highest cell count, but also supported multi-functional traits (phosphate and zinc solubilization, ammonia and IAA production) for 150 days. So, these bioformulations (WB5 and RB5 amended with 15mM trehalose and 0.1% CMC) along with charcoal-based consortium biofertilizer recommended by PAU, were further assessed for seedling emergence and growth of wheat (PBW826) under axenic conditions. Results revealed that these bioformulations were found suitable for seedling emergence and growth of wheat, where RB5 and WB5 with 15mM trehalose exhibited maximum emergence and growth of wheat seedlings. It can be deciphered that RB5 and WB5 amended with 15mM trehalose can be further used as a potential media formulation to develop a low-cost liquid consortium biofertilizer on the condition of their equivalent performance under pot and field evaluation for different crops.
Description
Keywords
Citation
Akashdeep (2024). Bioprocessing of cereal bran for production of low-cost liquid consortium biofertilizer (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.