Efficacy of low temperature tolerant plant growth promoting rhizobacteria on symbiotic efficiency, growth and yield of lentil (Lens culinaris L. Medikus)
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Date
2014
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Punjab Agricultural University Ludhiana
Abstract
A total of 35 isolates of rhizobacteria were isolated at 10 and 15°C from 20 soil samples taken from different locations and were characterized as belonging to genera Bacillus (23), Pseudomonas (11) and Serratia (1). IAA production ranged from 3.2-19.3 μg/ml and 7.6-20.4 μg/ml in the presence of tryptophan at 10 and 15°C respectively. Isolates J-6 was found to be strong producer of HCN at 10°C, however, at 15°C J-17 was the highest producer. Maximum P-solubilization index was shown by isolate J-14 (5.5 cm) followed by J-17 (5.4 cm) and J-18 (5.3 cm). At 15°C was shown by isolate J-5 (87.4 mg/100ml) showed highest P-solubilization followed by J-17 (77.2 mg/100ml). However, none of the isolates could solubilize phosphorus at 10°C. At 15°C maximum siderophore production was observed with isolate J-18 as evident by the halo on CAS agar and highest catechol type siderophore was produced by J-17 (606.1 µg/ml) followed by J-18 (595.1 µg/ml). However as in case of P-solubilization no siderophore production was observed at 10°C. Field experiment was conducted to examine the effect of co-inoculation of Rhizobium with PGPR strains (J-3, J-17, J-18 and J-30) for growth, symbiotic parameters and yield in lentil variety (LL-931). Coinoculation showed a significant increase in nodule number, nodule dry weight, plant and root dry weight, chlorophyll and leghaemoglobin content over Rhizobium alone. Application of PGPRs J-3, J-17, J-18 and J-30 along with Rhizobium further enhanced the grain yield (1.8, 4.4, 3.1 and 1.4%) over Rhizobium inoculation alone.
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Keywords
Microbiology, Lens culinaris L. Medikus, Biochemistry, PGPR, plant growth, rhizobacteria, lentil, Efficacy of low temperature