Management of botrytis gray mold (Botrytis cinerea Pers. ex. Fr.) of chickpea through host resistance and fungitoxicants
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Date
2019
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Punjab Agricultural University, Ludhiana
Abstract
Botrytis gray mold caused by Botrytis cinerea is one of the most destructive diseases of chickpea.
Maximum disease was observed in seedling inoculated from 5th to 25th February under 100
percent shade condition and disease development was more on PBG 5. Evaluation of chickpea
inter-specific derivative lines (115) and germplasm (68) revealed that none of the line possessed
high degree of resistance. Six inter-specific derivative lines (9054, 9056, 8007, 8018, 8022 and
8026) and two germplasm lines (GL-93011and GL-98010) showed moderately resistant reaction.
Out of forty seven accessions of wild Cicer species, sixteen accessions (C. judaicum ILWC-95,
C. judaicum ILWC-182, C. judaicum ILWC-185, C. judaicum ILWC-17148, C. reticulatum
ILWC-237, C. reticulatum ILWC-141E, IC-525691, IC-525197, EC-366339, EC-366342, C.
pinnatifidum ILWC-225, C. judaicum ILWC-223, C. pinnatifidum ILWC-261, C. pinnatifidum
ILWC-0, C. pinnatifidum ILWC-263 and C. pinnatifidum ILWC-212) gave resistant reaction. Out
of twenty seven lines derived from the cross of three parents (C. pinnatifidum 188, ICCV 96030
and PBG 1), only three lines (10008, 10009 and 10011) showed C. pinnatifidum 188 specific
segment and moderately resistant reaction. These three lines were flanked by primers GA-22,
GA-24, GA-105, NCPGR-127 and NCPGR-141. Testing of fungitoxicants by poison food
technique (in vitro) showed that maximum per cent growth inhibition of 78.66 and 84.54
respectively was found in azoxystrobin + difenconazole 325 SC even at low concentration of 1
and 5 ppm respectively. Next best fungitoxicant propiconazole 25 EC gave more than 90 percent
inhibition at 50 ppm. Similar results were achieved when these fungitoxicants were evaluated for
sporulation at their respective concentrations. ED50 value of 1 μl ml-1 was achieved in
azoxystrobin + difenoconazole, propiconazole and carbendazim. Non-systemic fungitoxicants
were found to be non-effective against the pathogen. Azoxystrobin + difenoconazole 325 SC
@0.1 percent with disease reduction of 88.68 per cent over control with per cent disease index of
7.72 as compared to check (68.24%) was followed by propiconazole 25 EC @ 0.1 percent disease
reduction of 85.85 per cent over control with per cent disease index of 9.65 per cent.
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