Standardization of Seed Testing Procedures in Some Medicinal Plants
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Date
2016-06
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University of Agricultural Science, Dharwad
Abstract
Study on four medicinal plant species viz., Mucuna pruriens, Phyllanthus emblica, Putranjiva roxburghii and Santalum album was undertaken to standardize media and temperature required to test for seed germination in the laboratory of Department of Seed Science and Technology and Seed Unit, University of Agricultural Sciences, Dharwad. The experiment was comprised of temperature ranges involving four constant (15, 20, 25 and 30 0C) and three alternate (15-25, 15-30 and 20-30 0C) temperatures and three media i.e., between paper (BP), top of paper (TP) and sand medium. Further experiment was also conducted to find out the effect of pre-sowing seed treatment on field emergence of four medicinal plant species in Saidapur Farm, UAS, Dharwad.
Among the different temperatures and media studied, maximum germination (90.88 %) was recorded in Mucuna pruriens at 30 0C temperature in BP method. The first and final counts were fixed on 4th and 8th day, respectively. In BP method at constant temperature of 30 0C Phyllanthus emblica recorded maximum germination (52.19 %). The first and final counts were fixed on 5th and 11th day, respectively. At constant temperature of 30 0C in BP method the highest germination (47.75 %) was recorded in Putranjiva roxburghii. The first and final counts were fixed on 15th and 24th day, respectively. In Santalum album the highest germination (23.95 %) was observed in sand medium at constant temperature of 30 0C. The first and final counts were fixed on 23rd and 37th day, respectively.
Among the seed treatments maximum germination (82.00 %) was recorded in the seed treated with GA3 (50 ppm) in Mucuna pruriens. In Phyllanthus emblica the highest germination (38.00 %) was recorded in the seeds treated with GA3 100 ppm + BAP 50 ppm. The seeds dipped in conc. H2SO4 for 10 minutes recorded highest germination (28.00 %) in Putranjiva roxburghii. In Santalum album highest germination (16.00 %) was recorded in the seeds treated with GA3 500 ppm.