In-vitro and in-vivo TLR-7 mRNA levels in response to lentogenic and velogenic pathotypes of Avian Avulavirus-1
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Date
2020-02
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Abstract
Transcriptional studies in response to Avian Avulavirus-1 (AAv1) have gained importance in the recent
past and have provided insight towards disease pathogenesis in chickens. The present study was focused
towards elucidation of TLR-7 mRNA levels in response to D58 (lentogenic vaccine strain) and D162 (velogenic
isolate) isolates of AAv1 in chicken embryo fibroblast cells (CEF) and chicken spleen at 1, 2, 3, 4 and 5dpi.
The mRNA levels were determined by real-time PCR using SYBR-green chemistry. The TLR-7 mRNA levels
were significantly up regulated to 18.90+0.03 in CEF cells and 12.33+0.02 in chicken spleen in response to
lentogenic D58 at 1dpi. Similarly, in response to velogenic D162, TLR-7 mRNA levels were significantlyunregulated to 52.40+0.03 in CEF cells and 34.62+0.02 in chicken spleen. Later, in both CEF cells and
chicken spleen, the mRNA levels of TLR-7 gradually declined from 2dpi in response to both lentogenic
D58 and velogenic D162. TLR-7 has been reported to be a protein that plays specific role in detecting single
stranded viral components and stimulating pro-inflammatory and anti-viral immune response. In the present
study, the significantly up regulated levels of TLR-7 mRNA at 1dpi confirms the fact that TLR-7 levels
were induced so as to stimulate necessary pro-inflammatory and anti-viral immune response against AAv-1.
Further, it was observed that the virulence of AAv-1 pathotype also affected TLR-7 mRNA levels. This shows
that TLR-7 mRNA levels vary with varying virulence of AAv1.
Description
TNV_20thMVC_PP_Feb-2020_PA57
Keywords
Veterinary Science