Biodiesel production from lipid generating microalgae

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dc.contributor.advisorAnand, R.C.
dc.contributor.authorNarula, Amrita
dc.date.accessioned2017-06-03T04:40:18Z
dc.date.available2017-06-03T04:40:18Z
dc.date.issued2014
dc.description.abstractIn the present scenario, the future of crude oil looks quite bleak as it may lead to all oil resources coming close to a moribund one day. Moreover, consumption of present petroleum sourced fuels at the rocket speed rate has also led to various environmental problems. One of the major concern is global warming. To make a dent in global warming, bioenergy must be generated at a very high speed. Microalgae may be the best option to produce bio-energy at rates high enough to replace a substantial fraction of fossil fuel used by our society. The primary objective of this study was to isolate lipid generating microalgal isolates for biodiesel production. The microalgal water samples were collected from ponds of different locations of eight districts of Haryana including Hisar, Rohtak, Fatehabad, Sirsa, Panipat, Karnal, Ambala and Kurukshetra. The samples were analysed for pH, EC, salinity, turbidity, total nitrogen, phosphorous and potassium. The microalgal samples were enriched in the Bold’s Basal medium and incubated at 23 ±10C, 50-55% humidity with 16:8 hours light: dark photoperiod for 21 days. A total of twenty four microalgal isolates were obtained after purification. Using Nile red staining technique microalgal isolates HMA-2 and FMA-2 were selected for further study and grown in four media of different chemical composition viz., Soil extract, Chu-13, Kuhl and Bold’s Basal medium. The microalgal isolates HMA-2 and FMA-2 produced maximum biomass (0.21 and 0.29g/l ) in Bold’s Basal medium whereas lipid content (29.6 and 25.2%) was maximum in Kuhl medium when grown at 23 ±10C, 50-55% humidity with 16:8 hours light: dark photoperiod for 21 days. Among the inorganic nitrogen sources tested potassium nitrate (original source) resulted in biomass accumulation of 0.12 and 0.19 g/l with 29.4 and 25.2% lipid content in the microalgal isolates HMA-2 and FMA-2 respectively while among organic nitrogen sources, peptone supplementation showed maximum lipid content of 38.2 and 22.1% from 0.04 g/l and 0.10 g/l biomass respectively. Similarly, among the carbon sources, glucose had been found to be a better supplement as it produced biomass of 1.59 and 1.21 g/l with 57.6 and 50.2% lipid content in microalgal isolates HMA-2 and FMA-2 respectively. Lipid and biomass production studies at different pH, temperature and salinity indicated biomass production of 0.14 g/l having 32.6% lipid at pH 8, 0.23g/l biomass having 42.4% lipid at 250C and 0.07g/l biomass containing 61.4% lipid on 20 g/l sodium chloride supplementation in microalgal isolate HMA-2 whereas in isolate FMA-2, 0.13, 0.21 and 0.11g/l biomass containing 26.1, 40.2 and 36.2% lipid was obtained under similar conditions respectively. Transesterification of algal oil of microalgal isolate HMA-2 using NaOH as catalyst and methanol resulted in biodiesel production to the tune of 9%.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810014966
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subMicrobiology
dc.subjectlipids, productivity, biomass, inorganic acid salts, biological development, biodiesel, sampling, irrigation, oils, biofuelsen_US
dc.these.typePh.D
dc.titleBiodiesel production from lipid generating microalgaeen_US
dc.typeThesisen_US
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