Identification of Molecular Markers Associated With Sterility Mosaic Disease Resistance in Pigeonpea [Cajanus cajan (L.) Millsp.]
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Date
2012
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Publisher
UAS, Dharwad
Abstract
Pigeonpea Sterility Mosaic Disease (PSMD) caused by Pigeonpea Sterility
Mosaic Virus (PPSMV) and transmitted by Eriophyid mite (Aceria cajani
Channabasavanna) is an important disease of pigeonpea. Inheritance of PSMD
resistance was studies in two crosses: in Gullyal white x BSMR 736 cross, out of 325
F2:3 families field evaluated, 55 were resistant and 270 were susceptible, while, in ICP
8863 x BSMR 736 cross, out of 225 F2:3 families field evaluated, 38 were resistant and
187 were susceptible to PPSMV with segregation ratios (susceptible: resistant) of
270:55 (P 0.05) and 187:37 (P 0.05) respectively, which is comparable to 13
(susceptible):3 (resistant) ratio. Hence, inheritance of PSMD resistance was found to be
controlled by two genes, SV1 and SV2, with inhibitory gene interaction both the crosses.
The parents of the crosses were tested with 300 RAPD and 20 SSRs markers and
in all 95 and 3 amplicons respectively recorded polymorphism. Bulked Segregant
Analysis (BSA) revealed IABTPPAK-19, IANTPPN-18 and IABTPPL-2 RAPD
markers association with susceptibility and IABTPPN-7 association with both
resistance and susceptibility in coupling-repulsion: these markers segregated in
expected 3:1 ration in F2 individuals of both crosses, while SSR marker, CcM0844 in
1:2:1 ratio. Single Marker Analysis (SMA) recorded 15, 8, 22, 20, 36, 5 and 3 per cent
phenotypic variance respectively for IABTPPAK-191295, IABTPPN-18827, IABTPPL-
2898, IABTPPN-7983, IABTPPN-7414 and CcM0844 markers. Association of these
markers with PSMD resistance/susceptibility was validated in 12 genotypes of known
reaction to PSMD. Linked RAPD markers were sequence characterised and prospected
for Sequence Characterised Amplicon Regions (SCAR) markers; one marker, FlnthN-
18, was found co-segregating in Gullyal white x BSMR 736 cross in 3:1 ratio. Single
Nucleotide Polymorphisms (SNPs) were identified in three out of five linked RAPD
amplicons. Further, field evaluation of lines from both crosses lead to at a subset which
is resistance to PSMD with a potential breeding value.