STUDIES ON STREPTOZOTOCIN INDUCED DIABETES IN RATS AND ITS AMELIORATION BY POMEGRANATE JUICE AND PEEL EXTRACT
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR-585401
Abstract
The present study was undertaken to evaluate the antidiabetic effect of pomegranate
juice and peel extract individually and in combination with metformin in STZ induced
diabetes in rats for a period of 45 days. The study included seven treatment groups
comprising of ten albino wistar rats each, randomized with minimal variation in initial body
weights. The various groups in this study included normal control (Group I (NC)), diabetic
control (Group II (DC)), diabetic rats treated with metformin at the rate of 500 mg/kg bw
(Group III (MF)), diabetic rats treated with pomegranate fresh juice at (1ml/day) Group
IV(PJ)), diabetic rats treated with pomegranate peel extracts (at 100mg) (Group V (PPE)),
diabetic rats treated with pomegranate fresh juice (1mL) + pomegranate peel extracts
(100mg) at 50 per-cent dosage and metformin (half dose) Group-VI (PJ+PPE+MF 50%),
diabetic rats treated with pomegranate fresh juice (0.5mL) + pomegranate peel extract
(50mg) at 50 per-cent dosage and metformin (half dose) Group-VII (PJ+PPE @ 50%
+MF 50%). The results showed that there was a significant increase (p ≤ 0.05) in the mean
glucose values in diabetic rats when compared to normal control rats. Similarly, the mean
(± SE) serum glucose, triglyceride, cholesterol, ALT, AST level were significantly (p ≤
0.05) higher and in RBC, Hb and platelets values were significantly (p ≤ 0.05) lesser in
diabetic control animals compared to the normal control animals. All the antioxidant
enzymes (CAT, SOD and GPx) were markedly reduced in diabetic control (Group II). The
treatment groups treated with metformin, pomegranate juice and pomegranate peel extract
(Group III to VII) individually or in combination showed a significant improvement in all
the parameters compared to diabetic control by the end of 45th day. However, it could be
inferred that metformin substantially alleviated the effects of STZ in diabetic rats compared
to all the treatment groups. Pomegrenate peel extract was observed to have marginally
better antidiabetic effects compared to pomegranate juice and among the combination
treatment groups (VI and VII) there was no dose dependent and synergistic effect observed.
Keywords: Pomegrenate peel extract (PPE), Pomegrenate juice (PJ), Metformin, Catalase
(CAT), Superoxide dismutase (SOD) and Glutathione peroxidase (GPx)