Development Of BHK 21 Cell Culture Adapted Peste Des Petits Ruminants (PPR) Virus Vaccine
| dc.contributor.advisor | Chandran, Daniel Joy | |
| dc.contributor.advisor | Balachandran, C | |
| dc.contributor.advisor | Raj, G. Dhinakaran | |
| dc.contributor.advisor | Kirubaharan, J. John | |
| dc.contributor.author | John, Koshy | |
| dc.contributor.author | TANUVAS | |
| dc.date.accessioned | 2016-05-27T14:54:07Z | |
| dc.date.available | 2016-05-27T14:54:07Z | |
| dc.date.issued | 2006 | |
| dc.description.abstract | The present study was undertaken to develop a BHK 21 cell culture adapted Peste des petits ruminants (PPR) virus vaccine. The PPR vaccine virus AR 87 adapted in Vero cell line monolayer was propagated in BHK 21 cell line monolayer and BHK 21 suspension cell line (Razi, Indian Immounological, Hyderabad) by giving fifteen and five serial passages respectively. The identification of the PPR vaccine virus adapted in BHK 21 cell line monolayer and BHK 21 suspension cell line carried out by HA and VNT and further confirmed with S-ELISA. The peak titre of PPR vaccine virus in BHK 21 cell line monolayer at 5 th , 10 th , 15 th and 1 st , 2 nd , 3 rd , 4 th and 5 th passage levels in BHK 21 suspension cell line at 72 hrs post infection were 8.23 log 10 , 8.41 log 10 , 8.54 log 10 , 9.22 log 10 , 9.31 log 10 , 9.50 log 10 , 9.66 log 10 and 9.82 log 10 TCID 50 / 0.1ml respectively. The standardization of the PPR vaccine virus adapted in BHK 21 suspension cell line were carried out by production of master seed virus, production of working seed virus, purity test, safety test and potency test. The PPR vaccine virus AR 87 adapted in BHK 21 suspension cell line was tested for potency and efficacy in sheep and goats. All thevaccinated animals were screened for seroconversion by HI, SNT and C- ELISA and produced sero conversion. The field and comparative study of both the PPR vaccine virus AR 87 adapted in BHK 21 suspension cell line and PPR vaccine virus AR 87 adapted in vero cell line monolayer were tested in sheep and goats. Serum samples were collected on day 0 and 21 st day post vaccination and screened for seroconversion by HI, SNT and C–ELISA and the titre compared between the two vaccines. The PPR vaccine developed in BHK 21 suspension cell line was found to give a good antibody response compared to PPR vaccine developed in vero cell line. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/66394 | |
| dc.language.iso | en | en_US |
| dc.publisher | Tamil Nadu Veterinary and Animal Sciences University | en_US |
| dc.sub | Veterinary Microbiology | |
| dc.subject | PPR | en_US |
| dc.subject | HA | en_US |
| dc.subject | VNT | en_US |
| dc.subject | HI | en_US |
| dc.subject | SNT | en_US |
| dc.subject | S-ELISA | en_US |
| dc.subject | C-ELISA | en_US |
| dc.these.type | Ph.D | |
| dc.title | Development Of BHK 21 Cell Culture Adapted Peste Des Petits Ruminants (PPR) Virus Vaccine | en_US |
| dc.type | Thesis | en_US |