Morpho-physiological and biochemical response of rabi sorghum genotypes to moisture stress under late sown rainfed condition.

dc.contributor.advisorAmarshettiwar, Dr. S. B.
dc.contributor.authorBOBADE, PRATIK NAGESH.
dc.date.accessioned2022-12-31T22:08:05Z
dc.date.available2022-12-31T22:08:05Z
dc.date.issued2019-09-13
dc.descriptionThe present investigation was carried out over 2 season (rabi 2015-2016, rabi 2016-17,) in which 13 genotypes of rabi sorghum were used for evaluation by using Randomized Block Design at the field of Sorghum Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, to study their morpho-physiological, biochemical parameters and yield of stress tolerant genotypes.en_US
dc.description.abstractThe present investigation on “Morpho-physiological and biochemical response of rabi sorghum genotypes to moisture stress under late sown rainfed condition” was carried out during rabi 2015-16 and 2016-17, at Sorghum Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. To estimate the morpho-physiological, growth and biochemical parameters associated with moisture stress tolerant under late sown rainfed condition and finding association among these all parameters for stress tolerant through correlation with yield for further study. The experiment was laid out in randomized block design, replicated thrice with thirteen genotypes (treatments) viz., M 35-1 (c), Phule Anuradha (c), Ringni (c), CSV-22R, CSV-26R, CSV-29R, Parbhani Moti, PKV-Kranti, Phule Maulee, Elongvan-19, Elongvan-42, Elongvan-227, Elongvan-277. Sowing was done under late condition by dibbling method on 21st November 2015 and 2016. Gap filling was done and plant protection measures were applied when necessary. Periodic observations were recorded on morpho-physiological parameters viz., leaf area plant-1, total dry matter, RWC, CTD, CCI and stomatal frequency at 30, 60 and 90 DAS with plant height at 30, 60, 90 DAS and harvest. Different growth parameters viz., RGR, CGR and AGR at 30-60 and 60-90 DAS while, LAI, SLW and number of green leaves plant-1 at 30, 60 and 90 DAS whereas, biochemical parameters like leaf proline content, CSI and nitrogen content in leaves were recorded at 60 and 90 DAS, nitrogen, protein and micronutrients content in seed were estimated after harvest and periodic soil moisture content was noted in thirty days interval upto harvesting. Observations on yield and yield contributing parameters were also recorded per replication and genotype (treatment) wise at harvest. Morpho-physiological, growth and biochemical parameters were correlated with grain yield. In the present investigation results revealed that genotype CSV-29R recorded significantly maximum leaf area plant-1 (21.85 dm-2), TDM plant-1 (108.75 g), RWC (65.85%), CTD (1.35oC) and CCI (39.20) at 90 DAS and abaxial (156.50 mm-2), adaxial stomatal frequency (132.0 mm-2) at 50 % flowering stage. Whereas, plant height-1 an maximum under CSV-26R (212.29 cm) at harvest, Among the genotypes, CSV-29R exhibited more RGR (0.34 g g-1 day-1), CGR (34.114 g m-1day-1), AGR (2.303 g day-1) at 60-90 DAS, LAI (3.34), SLW (1.23 g dm-2) and number of green leaves plant-1 (9.83) at 90 DAS than other genotypes and best check M 35-1. While, genotypes E-19, Phule Maulee, E-227, E-277 and PKV-Kranti also performed better for various morpho physiological and growth parameters. In biochemical analysis, the genotypes CSV-29R and E-19 at 90 DAS rewarded significantly higher proline content (4.38 and 23.40 µ mole g-1 FW), CSI (0.47 and 0.43%) and leaf nitrogen content (2.47 and 2.42%) respectively. Nitrogen content in seed (2.25 and 2.31 %), protein content in seed (14.06 and 13.05 %) and micro nutrients like, Zinc (17.20 and 15.93 mg kg-1) recorded maximum in the genotypes CSV-29R and E-19 respectively, while other seed micro nutrients i.e., Mn (5.68 and 5.45mg kg-1), Fe (46.43 and 44.25 mg kg-1) and Copper (1.80 and 1.65 mg kg-1) recorded significantly highest in genotypes E-19 and CSV-29R respectively, than other genotypes and checks at harvest. Phule Anuradha (check) and CSV-29R were found earlier in physiological maturity. In relation with yield and yield attributes, genotype CSV-29R and E-19 noted significantly highest panicle length (18.17 and 16.63 cm), panicle diameter (7.33 and 7.20 cm), earhead exertion (74.7 and 70.5 %), number of grains panicle-1 (1544 and 1478), grain weight plant-1 (22.95 and 20.67 g), test weight (32.62 and 30.62 g), grain yield (2393 and 2090 kg ha-1), biological yield (6308 and 5844 kg ha-1) and harvest index (37.96 and 35.76 %) respectively, than other genotypes and best check M 35-1. Among the three checks, M 35-1 recorded best in performance on the basis of all traits under study than other check genotypes viz. Phule Anuradha and Ringni in moisture stress under late sown rainfed condition. Current study was also erudite for the optimization of appropriate concentration of PEG for screening the genetic resource of rabi sorghum for further detailed studies. Various osmotic stress levels had substantial effects on germination and early vegetative growth traits of rabi sorghum genotypes. At highest concentration (-0.066 MPa) of osmotic (PEG) stress condition, genotypes M-35-1, E-277 and E-227 showed positive result for shoot length, root length and seedling dry weight. Germination per cent, seedling vigour, shoot length, root length, seedling dry weight and stress tolerance index also decreases with increase in osmotic stress. Considering the correlation coefficient on grain yield found positive and significant correlation with all morpho physiological, growth and biochemical parameters except plant height plant-1. While, yield has negative significant correlation with days to 50 % flowering and physiological maturity. Traits under study viz., may be used in the breeding programme for the development of moisture stress tolerant genotypes under late sown rainfed condition for changing climatic scenario.en_US
dc.identifier.citationBOBADE, PRATIK NAGESH. (2019). Morpho-physiological and biochemical response of rabi sorghum genotypes to moisture stress under late sown rainfed condition. Department of Agricultural Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Ph. D. (2019). Print. xxv, 265p. (Unpublished).en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810190951
dc.keywordsCrops, Field Crops, Sorghum, Sorghum bicolor, Leaf area, Leaf area index, Dry matter, Chlorophyll, Germination, Leaves, Roots, Photosynthesis, Abiotic Stress, Drought, Morpho-physiological Parameters, Biochemical Parameters, Sorghum Yield, Crop Growth Rate, Research, Field Experimentation, In Vitro Experimentation, Experimental Design,en_US
dc.language.isoEnglishen_US
dc.pagesxxv, 265p.en_US
dc.publisherDr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.en_US
dc.research.problemIn the present research the researcher tried to study the effect of moisture stress under rainfed condition on morpho-physiological, biochemical traits and germination ability in rabi sorghum genotypes also study the correlation coefficients of important morpho-physiological and biochemical traits with yield.en_US
dc.subPlant Physiologyen_US
dc.themeThe present research deals with to study the effect of moisture stress under rainfed condition on morpho-physiological and biochemical traits in rabi sorghum genotypes and correlation with yield by using randomized block design and to test germination ability of sorghum genotypes under stress using PEG 6000 by using completely randomize design.en_US
dc.these.typePh.Den_US
dc.titleMorpho-physiological and biochemical response of rabi sorghum genotypes to moisture stress under late sown rainfed condition.en_US
dc.typeThesisen_US
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The present investigation was carried out over 2 season (rabi 2015-2016, rabi 2016-17,) in which 13 genotypes of rabi sorghum were used for evaluation by using Randomized Block Design at the field of Sorghum Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, to study their morpho-physiological, biochemical parameters and yield of stress tolerant genotypes.
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