IN VITRO REGENERATION STUDIES OF “SWEET ORANGE”Citrus sinensis L.Osbeck
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Date
2022-12-13
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University of Agricultural Sciences, Bangalore
Abstract
Sweet orange (Citrus sinensisL.Osbeck) is widely cultivating member of the citrus genus with immense nutritional values. The conventional propagation of sweet orange crop is being limited due to bud transmittable diseases and low success rate, therefore the present investigation was undertaken to develop a protocol for in vitro regeneration and production of multiple disease free shoots of Sweet orange.The nodal segments and seeds were used as explants. The surface sterilized explants were cultured on full strength MS media supplemented with different concentration and combination of plant growth regulators namely BAP, Kinetin and NAA. Better responses were observed with different combinations of growth regulators. The combination of BAP 1.0 mgL-1 + Kinetin 2.0 mgL-1 produced maximum number of shoots of 5.9 at 90 days after shoot initiation when nodal segments were used as explants. Whereas, for the seed explants maximum number of shoots (3.8) at 60 days after seed germination was obtained in BAP 0.5 mgL-1 + Kinetin 0.5 mgL-1. The nodal segment explants placed in different concentration of NAA did not show any shoot growth. Use of NAA for in vitro regeneration of sweet orange is not recommended either when used individually or in combination with other growth regulators, it produced only callus which did not regenerate further. The in vitro regenerated shoots were
transferred to half strength MS media contains rooting hormones namely IBA and NAA at different concentration and IBA at 1.5 mgL-1 showed maximum number of roots as well as longer roots.