Effect of Semen Diluents, Dilution Rates and Storage Periods on Live and Abnormal Spermatozoa of Pearl Guinea Fowls
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Date
2016
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Abstract
Background: Guinea fowls are neglected poultry with lower fertility rates compared to other avian species. Semen quality is one of the
majordeterminants of fertilitywhich deteriorates soon aftercollection.Therefore,theability ofdifferent semen extenders in maintaining
the livability and morphology of Guinea fowl spermatozoa under short term storage was studied. Materials and Methods: A total of
16 male Guinea fowls were housed in individual cages and was trained for semen collection by abdominal massage technique. Pooled
semen samples werethen diluted with two different semen extenders namely the beltsville poultry semen extenderand instruments for
veterinary medicine poultry semen preservation media each in the ratio 1:2 and i:3.The diluted samples werethen stored at 5°C and the
percent spermatozoa livability and abnormality were assessed at 0, 3 and 6 h of storage. Results: Based on the study, the livability and
morphologically normal spermatozoa were maintained above 80% with both the extenders until 6 h ofstorage. With respect to dilution
rates, the semen in lower dilution had shown a higher percentage of live and morphologically normal spermatozoa irrespective of
extenders and storage periods. The percent dead spermatozoa and abnormal spermatozoa significantly increased with increasing storage
periods. Conclusion: Based on the results, it was concluded that the Guinea fowl semen may be extended with either ofthe diluents at
lower dilution rates and the diluted semen must be inseminated within 3 h after collection which may have positive impact on fertility
by artificial insemination.
Description
TNV_AJAVA_2016_11(7)411-416
Keywords
Veterinary Science