Exploration of Hepatoprotective and Antioxidant Properties of Earthworm Extract and Trigonella Foenum-Graecum Seed Extract (TSE) Against Paracetamol Intoxication in Rats
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Date
2014
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Publisher
TANUVAS, Chennai
Abstract
Trigonellafoenum-graecum seed extract and paste of earthworm
Eudriluseugeniae in paracetamol induced hepatotoxicity in rats. The effects of the
test drugs were compared to the standard drug silymarin.
Prior to the animal study, in vitro antioxidant capacity of the test
drugs and the in silicobinding ability of the components of the test drugs with the
enzyme CYP 2E1 were done. Albino rats of Wistar strain were utilized in the
study. The test drugs (100 and 300 mg/kg b.wt) were administered for a period of
seven days and then toxic dose of paracetamol (3g/kg b.wt) was administered on
eighth day. One group was treated with the standard drug, silymarin at the dose
rate of 150 mg/kg b.wt. All the rats were sacrificed on the ninth day. Serum
analysis for liver function, estimation of enzymatic and nonenzymatic antioxidants
of liver, CYP2E1 activity assay, Real time PCR for HemeOxygenase 1 (HO1)
gene and histopathology of liver were carried out at the end of the study.
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Keywords
Veterinary Science, Veterinary Pharmacology and Toxicology