Isolation and characterization of plant growth promoting salt tolerant bacteria from Suaeda fruticosa L. rhizosphere
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Date
2023-06
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CCSHAU, Hisar
Abstract
Soil salinity is one of the most severe environmental factors that limits the productivity of crops in arid or semi-arid regions around the world. The present investigation was undertaken to isolate and characterize plant growth promoting salt-tolerant bacteria. Rhizospheric soil samples were collected from Suaeda fruticosa halophyte in Hisar and were screened for plant growth promoting salt tolerant bacteria. Eighteen isolates were retrieved and analyzed for salt tolerance at various NaCl concentrations (2% to 10% NaCl). Twelve isolates were found salt tolerant at varied salt concentrations and evaluated for the plant growth promoting traits. All the salt tolerant isolates showed IAA production with maximum in isolates SA3, SR1, SR2, and SS1. Ammonia excretion was maximum in isolate SR1 and minimum in isolate SR5. Ten isolates showed phosphate solubilization and eight isolates showed Zn solubilization. All twelve salt tolerant bacterial isolates were found negative for siderophore production. Four isolates SA3, SR1, SS1 and SR2 with significant salt tolerance and PGP traits were characterized biochemically. Based on biochemical results, the tentative genus identified as Bacillus (SA3, SR1, and SR2) and Serratia (SS1). The effect of salt-tolerant PGP bacterial isolates on seed germination of wheat, pearl millet, mung bean, and chickpea under different salt concentrations was determined. By increasing the salt concentration, salt tolerant PGP isolates showed better germination than the control. However, isolates SA3 and SR1 showed maximum seed germination and were further identified by 16s rDNA gene sequencing. 16S rDNA gene sequencing of isolate SA3 showed 99.64% similarity with Bacillus safensis and SR1 isolate showed 99.78% similarity with Bacillus cereus.