TISSUE CULTURE STUDIES IN SAFED MUSLI {Chlorophytum borivilianum Sant and Fern.)
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Date
2007-08-31
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University of Agricultural Sciences GKVK, Banglore
Abstract
The present investigation on tissue culture studies in safed musli
(Chlorophytum borivilianum Sant and Fern.) was carried out during 2004-
05 at the tissue culture laboratory of Horticulture Department, College of
Agriculture, University of Agricultural Sciences, Dharwad. In the present
study, sub experiments were conducted by applying CRD design, in order
to find out the best explant and growth regulator concentration for shoot
and root morphogenesis of safed musli and also plantlets were assessed
for hardening under different growing conditions.
Four different explants were used in the investigation. Among them,
stem disc and shoot base explants on Murashigae and Skoog (MS) media
showed better response in terms of early initiation of culture, culture
establishment and number of shoots. Both leaf base and tuber explants
gave very poor response.
Among growth regulator concentration assessed for
standardization, MS medium containing 2 mg/1 BAP gave more number of
leaves, shoots and maximum shoot length. The high (+++) and intense
response of root proliferation was observed on full strength of MS
medium at 2"^ and 4*^ week respectively after inoculation with the
increasing concentration (2 to 3 mg/1) for indole-3-butyric acid (IBA) and
benzyl amino purine (BAP) at 2 mg/1.
Transferred safed musli plantlets established better under net
house condition compare to direct exposure into the field.
Micro propagation seems to be the best method compared with
other methods of multiplication. In safed musli in vitro method increased
the rate of multiplication while combating the problem of long tuber
dormancy to facilitate for growing were than one crop per year
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