TAURINE AS AN ADDITIVE IN ANDROMED EXTENDER FOR CRYOPRESERVATION OF GIR BULL SEMEN
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Date
2018-06
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JAU,JUNAGADH
Abstract
ABSTRACT
The present investigation on the “Taurine as an additive in andromed extender for cryopreservation of Gir bull semen” was undertaken at Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh,Gujarat. The study was carried out on four Gir bull (4-6 years) located at Cattle Breeding Farm J.A.U. Junagadh for a period of 6 weeks. Semen was collected using artificial vagina method once weekly for six weeks from each of the bulls. The semen was evaluated for physical characteristics and oxidative stress at, neat semen (pre-dilution), per-freeze and post-thaw stages of cryopreservation using different Taurine concentrations viz. 25mM Taurine, 50 mM Taurine, 75 mM Taurine and control was kept.
Onevaluation of 24 semen ejaculates (6 ejaculates from each bull) of neat semen, ejaculate volume (ml), sperm concentration (million/ml), mass motility (0-5 scale), individual motility (%), sperm viability (%), sperm abnormality (%), HOST reactive spermatozoa (%), and acrosome integrity were found to be 5.80± 0.41, 1267.63± 106.32, 3.88 ± 0.07, 85.83 ± 1.94,87.58 ± 0.42,10.87 ± 0.41, 83.00 ± 0.84 and 91.87 ± 0.32, respectively. There was a significant difference in volume and concentration of semen between the bulls while the difference was non-significant for the mass motility, individual motility, viability, abnormality, HOST reactive spermatozoa and acrosomal integrity.
The levels of oxidative stress parameters viz. lipid peroxidation and glutathione reductase were 51.14 ± 0.18 μmol/ml and 32.24± 0.64 U/L, respectively. The difference was non-significant among the bulls for the glutathione reductase, while significant (P<0.05) difference was found for the lipid peroxidation.
Individual motility, mass motility, viability,HOST reactive spermatozoa, glutathione reductase and acrosomal integrity were positively correlated with each other.
At pre-freeze stages of cryopreservation, 50mM Taurine group had value of 70.20 ± 1.22, 69.87 ± 3.09, 69.5 ± 1.14 and 86.54 ± 0.81 percent for individual motility, sperm viability, HOST reactive sperm and acrosomal integrity respectively, value were significantly (P<0.05) higher as compared to that of the 25 mM Taurine, 75 mM Taurine and control groups.Mean per cent sperm abnormality was 15.37 ± 0.89 in 50mM Taurine group at pre-freeze stages of cryopreservation, which were significantly (P<0.05) lower as compared to that of the othergroups.
Mean Malondialdehyde (MDA) levelwere30.74 ± 0.55μmol/ml in 50mM Taurine group at post-thaw stages of cryopreservation, and were significantly (P<0.05) lower as compared to that of the other groups.Mean glutathione reductase levels in 50mM Taurine group were 64.76 ± 0.78 U/L at pre-freeze stages of cryopreservation, and were significantly (P<0.05) higher as compared to that of the other groups.
At post-thaw stages of cryopreservation, 50mM Taurine group had value of 62.70 ± 0.42, 65.66 ± 0.58, 61.87 ± 0.76,and 84.50 ± 0.64 percent for individual motility, sperm viability, HOST reactive sperm and acrosomal integrity respectively, value were significantly (P<0.05) higher as compared to that of the 25 mM Taurine, 75 mM Taurine and control groups.Mean per cent sperm abnormality was 20.29 ± 0.73 in 50mM Taurine group at post-thaw stages of cryopreservation, which were significantly (P<0.05) lower as compared to that of the othergroups.
Mean Malondialdehyde (MDA) levelwas 15.95 ± 0.74μmol/ml in 50mM Taurine group at post-thaw stages of cryopreservation, and were significantly (P<0.05) lower as compared to that of the other groups.Mean glutathione reductase levels in 50 mM Taurine group were 80.25 ± 4.85U/L at post-thaw stages of cryopreservation, and were significantly (P<0.05) higher as compared to that of the other groups.
All of above physico-morphological and bio-chemical parameters were better in 50 mM Taurine group at pre-freeze and post-thaw stages of cryopreservation.
In conclusion, it was found that 50 mM is the most appropriate concentration of taurine as an additive in andromed extender to improve the semen quality parameters in Gir bull semen.
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VETERINARY SCIENCE & A.H.