Studies on macro and micro propagation in bamboo
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Date
2007-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present investigation entitled “Studies on Macro and Micro Propagation in Bamboo” was carried out at new site of Agroforestry Research Centre, Haldi, Pantnagar during June 2006 to May 2007 and in tissue culture laboratory of the Agroforestry Section, College of Agriculture, G.B. Pant University of Agriculture and Technology, Pantnagar.
Experiments were conducted to see the effect of age (one and two year old), growing condition (open intermittent misting and open no misting), position (basal, middle and top) in different seasons (June, August, October and March) on rooting and sprouting of culm cuttings of Bambusa balcooa and Dendrocalamus asper. The findings indicated that two year old culm cuttings collected from basal position in the intermittent misting condition gave best response in B. balcooa. In D. asper, one year old culm cuttings with above condition showed good results. The findings also indicated that rooting and shooting per cent of culm cuttings planted in different season showed response as March> June> August> October in both the species.
For micropropagation nodal buds from one year old B. balcooa and D. asper were taken as ex-plant. In B. balcooa for sterilization 0.1% Tween-20 (5 min), (1.0% Bavistine (5 min), 0.1% Streptomycin (4 min), 70% Ethanol (5 min), 1.0% NaOCl (8 min) and 0.1% HgCl2 (3 min)), for bud break (3.0 ppm BAP+ 1.0 ppm GA+ 0.5 ppm Kinetin), for proliferation of bud (4.0 ppm BAP+ 0.5 ppm Kinetin) and in D. asper 0.1% Tween-20 (5 min), (1.0% Bavistine (4 min)+ 0.1% Streptomycin (3 min)+ 70% Ethanol (5 min)+ 1.0% NaOCl (10 min)+ 0.1% HgCl2 (5 min)) for sterilization, (3.0 ppm BAP, 0.5 ppm Kinetin+ 0.5 ppm GA) for bud break, (5.0ppm BAP+ 1.0 ppm) for proliferation of bud were found to be best for this investigation. In vitro rooting studied using different auxins; 3 ppm NAA+2.5 ppm IBA and 4 ppm+ 2 ppm IBA were found to be best for B. balcooa and D. asper respectively.
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