In vitro and ex vitro rooting and hardening of crab apple (Malus baccata var. Himalaica)’
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Date
2017
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UHF,NAUNI
Abstract
ABSTRACT
In vitro rooting and hardening of Malus baccata var. Himalaica, Kashmir (crab apple) was attempted in the present studies. Rooting response was tested at IBA concentrations of 0.3,0.5,0.7 and 1 mg/l without and with dark treatment which resulted in highest root induction (71%) in dark treated shoots, though little callus was found. 1 and 1.5 g/l activated charcoal was supplemented with above concentration of IBA. All the treatments resulted in more than 54% rooting and highest rooting efficiency (95%) was found at 1 mg/l IBA and 1 g/l activated charcoal. While testing different levels of NAA (0.2-0.5 mg/l), 63-89% rooting response was obtained. However, basal stem portions revealed profuse callogenesis and roots seemed to emerge from callus. To counter this problem, these levels of NAA were supplemented with 1.5 g/l AC which resulted in 70-90% rooting efficiency and inhibited callus formation. During hardening of in vitro plants, it was found that rooted plantlets with callus survived less while without callus survived 77% in peat pots. On their transfer to soil containing potting mixtures after six weeks, 100 percent plants survived. Ex vitro rooting results showed that soaking of shoots in low IBA in dark before their transfer to potting mixture is better (62-85% rooting) as compared to high auxin dip.
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