In-vitro regeneration studies in strawberry (Fragaria x ananassa) cv. Winter Dawn
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Date
2023
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RPCAU, Pusa
Abstract
The strawberry (Fragaria x ananassa Duch.) is one of the most notable tart, sweet and juicy fruits in the world. It is the second-largest berry fruit produced globally, after grapes. As it spreads viral infection, the traditional method of strawberry multiplication by employing runners has some limitations. In order to create an effective technique for multiple shoots, root induction and proliferation and regeneration of strawberry plants using explants of runner tip and nodal segments, the current experiment was carried out.
The present investigation entitled “In-vitro regeneration studies in strawberry (Fragaria × ananassa) cv. Winter Dawn” was carried out during 2022-2023 in Plant tissue culture laboratory, SRI, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur (Bihar). The variety Winter Dawn was used for the present study. The two types of explants used were runner tip and nodal segments. The three experiments: sterilization, shooting protocol and rooting protocol were carried out. For sterilization, mercuric chloride and ethanol were used and their effects were investigated. The shoot regeneration study was carried out with different concentrations of BAP was employed at concentrations of 0.5, 1.0, 1.5, and 2.0 mg/l, Kinetin at 0.2, 0.4, 0.6, and 1 mg/l and GA3 at 0.2, 0.4, 0.6 and 1 mg/l. The rooting protocol was standardized using NAA and IBA at varying concentrations (0.5, 1.0, 1.5, 2.0 mg/l).
The runner tips were found to be better in every experiment carried out. BAP levels of 1.5 and 0.6 mg/l Kinetin exhibited better than any other combination in case of shoot regeneration and the medium enriched with NAA at 0.5 mg/l gave good results compared to other combinations.