Biochemical expression analysis of rice bacterial blight resistance genes, Xa38 and Xa23 at elevated temperature
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Date
2017
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Punjab Agricultural University, Ludhiana
Abstract
Bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important disease of rice causing significant yield losses. Currently, there are ten pathotypes of Xoo prevalent in Punjab that has diverse virulence spectra. During the current studies biochemical basis of resistance of two new R genes, Xa38 and Xa23 was showed along with four other genes/ cultivars. The biochemical expression of these six genes/cultivars was studied against five pathotypes of Xoo viz. PbXo-4, PbXo-7, PbXo-8, PbXo-10 and Sgr-1001. These pathotypes showed differential disease reaction on these R genes/ cultivars. It was observed that resistance level offered by Xa38, Xa23 and other R genes/ cultivars in this study is highly correlated with expression of these defence related enzymes viz. Peroxidase, Polyphenol oxidase and Phenylalanine ammonia lyase. It was further observed that expression of Xa38 and Xa23 was not influenced by temperature whereas Xa7 was more effective at high temperature and Xa4 was more effective at lower temperature. No change in aggressiveness of the re-isolated colonies were observed although genetic variations were observed in re-isolated colonies exposed to high temperature and incompatible interactions. The RAPD primer S118 was observed to be highly discriminatory.
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