Genomic variation in production linked candidate gene and its association with performance & egg quality traits and kinetics of antibody response to vaccines in Uttara & Kadaknath native chickens
Loading...
Date
2022-10
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G. B. Pant University of Agriculture and Technology, Pantnagar
Abstract
The present study was conducted to evaluate the growth, egg production, egg quality traits and kinetics of antibody response to NDV and IBDV vaccines of Uttara and Kadaknath chicken, their genotyping with respect to egg production associated gene ie. MTNRIC, to associate candidate gene polymorphism with production traits and to associate level of expression of candidate with egg production trait in Uttara and Kadaknath chicken. The data on body weights from day old to 40 weeks at four weeks interval, ASM, BW20, BW 32, BW40, EW32, BW40, EW40, EP32 and EP40 were recorded on 300 from two hatches pedigreed birds maintained at IPF, Nagla, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand. Data were analysed by LS ANOVA using mixed model, wherein sire was taken as random effect and hatch as fixed effect. The estimated least square means of CW, BW4, BW8,BW12,BW16 and BW20 were 36.99±0.14, 175.43±0.19, 460.46±0.18, 806.58±0.28, 1139.05±0.72 and 1334.31±6.70 g respectively.Sire had significant effect (P≤0.05) on CW, BW12 and highly significant effect (P≤0.01) on BW20. Hatch had significant effect (P≤0.05) on CW. Effect of sex of the bird was found be highly significant (P≤0.01) from CW up to 20-week.Heritability estimates were 0.18±0.14, 0.42±0.11, 0.01±0.10, 0.12±0.13, 0.05±0.11 and 0.68±0.25 respectively. Genetic correlation of BWwere ranged from -0.96±1.01 to 0.57±0.63 and phenotypic correlations were ranged from 0.16 to 0.96.In Uttara birds, the least squares means (LS-means) of ASM, BW20, BW32, BW40, EW32, EW40, EP32 and EP40 were 172.45 ± 0.30 days, 1313.73 ± 0.55, 1570.74 ± 0.57, 1588.77 ± 0.62, 50.95 ± 0.19, 54.89 ± 0.11, 52.06 ± 0.22 and 74.23 ± 0.19 g respectively. Sire had significant effect (P≤0.05) on ASM, BW32 (P≤0.01) and BW40 (P≤0.01). Hatch had significant effect (P≤0.01) on BW32, BW40 and EP40 (P≤0.05). Heritability estimates were 0.26 ± 0.21, 0.13 ± 0.18, 0.52 ± 0.27, 0.45 ± 0.25, Not Estimate, 0.18 ± 0.19, 0.12 ± 0.18 and 0.08 ± 0.17 respectively. BW20, BW-40, ASM, EW40 showed high heritability while EP40 showed medium heritability. In Kadaknath native chicken the estimated least square means of CW, BW4, BW8, BW12, BW16 and BW20 were 27.96±0.18, 177.84±1.23, 371.55±1.54, 593.34±2.04, 809.30±3.36 and 1281.09±1.14 g respectively.Sire had significant effect (P≤0.05) on CW and BW20.The least squares mean (LS-means) of ASM, BW20, BW32, BW40, EW32, EW40, EP32 and EP40 were 187.63±0.49 days, 1272.76±1.24 g, 1459.74±1.07 g, 1544.90±1.50 g, 42.11±0.20 g, 48.35±0.30 g, 31.51±0.26 eggs and 47.56±0.28 eggs, respectively. Sire had a non-significant effect on all the economic traits except BW20.Hatch was also found to have a nonsignificant effect on all the concerned traits. The heritability estimates of BW20, BW40, ASM, EW28 and EP40 were 0.81 0.39, 0.79 0.34, 0.24 ± 0.11, 0.66 0.24 and 0.31 ± 0.12, respectively. The heritability estimates of BW20, BW40, EW28 were high while ASM and EP40 had medium heritability. The heritability of EW40 could not be estimated.In Uttara and Kadaknaththe genetic and phenotypic correlation coefficients were positive and ranged from 0.83 ± 0.43 to 0.01 ± 0.18 and 0.16 to 0.96 in Uttara and 0.86 ± 0.12 to 0.07 ± 0.64 and 0.06 to 0.90 in Uttara and Kadaknath respectively, both with moderate to higher magnitudes for most of the traits. High genetic correlation was reported between adjacent body weights as compared to distant body weights. In Uttara birds, the least squares means (LS-means) of external egg quality traits via., EW32, EL, EWd, SI, SA, EV, SG and ESP were 50.95±0.19 g, 55.20±0.23 mm,, 40.65±0.23 mm, 72.70±0.28 %, 71.71±0.54 cm2, 47.68±0.19 cm3, 1.07±0.00 and 10.54±0.13 %, respectively. The least square mean ± standard errors of internal egg quality traits in Uttara native chicken were AWt ,YWt, AH, YH, AWd, YWd, EST, ESW, AI, YI, HU, ApH and Yp H were 29.00±0.16 g, 16.37±0.10 g, 6.01±0.03 mm, 16.18±0.10 mm, 69.79±0.19 mm, 38.80±0.09 mm, 0.39±0.001 mm, 5.48±0.05 g, 8.63±0.04 %, 39.40±0.32 %, 80.04±0.17 %, 8.58±0.04 and 6.43±0.01, respectively. In Kadaknath birds, the least squares means (LS-means) of external egg quality traits via., EW32, EL, EWd , SI, SA, EV, SG and ESP were 42.11±0.20 g, 45.1±0.01 mm, 33.07±0.01 mm, 76.39 ± 0.57 %, 74.71±0.34 cm2, 43.58±0.22 cm3,1.10 ± 0.00 and 10.15±0.05 %, respectively. The least square mean ± standard errors of internal egg quality traits in Uttara native chicken were AWt , YWt AH, YH, AWd, YWd, EST, ESW, AI, YI, HU, ApH and Yp H were 26.29 ± 0.49 g, 12.49 ± 0.09 g, 5.05±0.003 mm, 15.01±0.04 mm, 70.026±0.01 mm, 42.21±0.037 mm, 0.33±0.003 mm, 4.34 ± 0.04 g, 7.21 ± 0.02 %, 36.7 ± 0.08 %, 74.99 ± 0.69 %, 9.26±0.12 and 6.22±0.03, respectively. In the studied population of Uttara and Kadaknath, MTNRIC candidate genes was of polymorphic nature. MTNRIC Mbo/RFLP studies in Uttara birds revealed two alleles A and B with allelic frequencies of 0.79 and 0.21, respectively. The allelic frequencies were 0.59 and 0.41, respectively in Kadaknath birds. The association of egg-production linked MTNRIC candidate gene with egg production traits in Uttara and Kadaknath, the resultant genotypes had significant effect on ASM and EP40 (P<0.05) in case of Uttara native chicken. The birds genotyped as AA achieved ASM at the earliest and laid maximum eggs up to 32 weeks and 40 weeks. The ASM of birds genotyped as AA, BB and AB were 166.32±0.21, 172.54±0.11 and 176.23±0.09 days, respectively. The EP40 of AA and BB genotyped birds were significantly different from that of AB genotyped birds. In Kadaknath birds, the effect of MTNRIC genotype was significant on BW20 (P<0.05) and EP40 (P≤0.15). The highest BW20 was observed in birds with AA genotype as which was statistically similar to h t i w d e t a i c o s s a e b o t d n u o f o s l a s a w e p y t o n e g A A . e p y t o n e g B A h t i w s d r i b f o t a h t m o r f t n e r e f f i d t u b e p y t o n e g B B f o t a hth ighest egg production at EP32 and EP40. The estimated mean antibody titres (log2) against NDV and IBDV vaccine were estimated on 7 dpi (8.10±0.22), 14 dpi (7.89±0.18) and 21 dpi (7.92±0.16) for NDV and 28 dpi (8.29±0.14), 35 dpi (8.40±0.16) and 42 dpi (8.94±0.19) for IBDV respectively