MEDIA MANIPULATION FOR IN VITRO PLANT REGENERATION IN PEPPERMINT (Mentha piperita L.)
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Date
2007
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Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
In Jharkhand, it is grown to some extent in
Chotanagpur, Ranchi and Santhal Pargana regions.
Its essential oil is called peppermint oil and contains
menthol, menthofuron, methone, iso-methone iso-menthol, neomenthol,
neo-isomenthol etc. Another important substance is rosmarinic
acid. It is an ester of caffeic acid and 3, 4 dihydroxy phenylacetic acid
possessing antioxidant and antimicrobial activities. A lot of complex
medicines and phyto-preparations are made of mint and menthol.
Peppermint oil also has other applications for e.g. as a flavouring agent
in confectionary items, alcoholic drinks, dental creams, mouth washes,
soaps, chewing gums, candies etc. Its essential oils also have
antibacterial and antifungal properties.
The present study was undertaken to standardize protocol
for in vitro regeneration of this crop.
Surface sterilized shoot tips and nodes were inoculated on
Murashige and Skoog’s (M.S) medium either supplemented with
hormones i.e. 6-Benzylamino purine (B.A.P) and 6, Furfurylamino purine
(Kn) (single or incombination) or without hormones. Maximum bud
breaking was obtained in B.A.P 1.0 mg/l. In B.A.P 1.0 mg/l and 2.5 mg/l
maximum shoot multiplication was observed in case of nodes and shoot
tips. Maximum root induction were found in B.A.P 1.0 mg/l. Rooted
plants were transferred for hardening. The survival percentage was 90
per cent during hardening.
Description
MEDIA MANIPULATION FOR IN VITRO PLANT REGENERATION IN PEPPERMINT (Mentha piperita L.)
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