Assessment of low-cost media for production of liquid wheat consortium biofertilizer
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Date
2022
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Punjab Agricultural University, Ludhiana
Abstract
Different media formulations of wheat bran and vegetable waste (10%, 20%, 30%, 40% and 50%) were evaluated for growth of consortium biofertilizer cultures. The optical density (OD) enhanced gradually from 0 to 5th day followed by constant values onwards. Maximum optical density was recorded in the standard broth i.e., 0.73±0.06, 0.87±0.05 and 0.84±0.03 for Azotobacter sp., Bacillus sp. and Pseudomonas sp. respectively, where among the low-cost bioformulations, maximum OD was observed in 40% of wheat bran (WB) in case of Azotobacter sp. (0.63±0.03) and Bacillus sp.
(0.67±0.04), while 30% WB was found superior for Pseudomonas sp. (0.83±0.04). Observations revealed that wheat bran portrayed comparatively higher OD for all the bacterial cultures. The viability
of consortium biofertilizer cultures was then assessed on the 5th day of incubation where 40% WB exhibited the highest growth for Azotobacter sp. (8.15±0.12 log CFU/ml) and Bacillus sp. (8.77±0.03
log CFU/ml). However, in the case of Pseudomonas sp. 30% WB (8.73±0.21 log CFU/ml) had the highest viable cell density compared to standard broth i.e., 8.34±0.02 log CFU/ml, 8.84±0.40 log
CFU/ml and 9.86±0.20 log CFU/ml for Azotobacter sp., Bacillus sp. and Pseudomonas sp. respectively. Analysis of different liquid media formulations for indole acetic acid production, ammonia production, phosphate and zinc solubilization revealed that 40% WB followed by 30% WB were found to be the foremost media formulations where among the vegetable waste (VW) concentrations, 40% of VW exhibited the highest values, but were comparatively lower than that of the WB formulations. On the basis growth and multifunctional traits, the treatments were narrowed down to 30% and 40% of WB and 40% of VW and standard broth i.e., Jensen’s broth for Azotobacter sp. and nutrient broth for Bacillus sp. and Pseudomonas sp. Assessment for the seedling emergence, shoot and root length of wheat (PBW823) under axenic conditions showed 40% WB to be the most prominent media formulation significantly comparable to standard broth. Additionally, the viable population of consortium biofertilizer cultures was also monitored at monthly interval where gradual decline in the viable cell count for all the treatments in consortium biofertilizer cultures was observed. Standard broth
was found to be desirable at 1 month interval followed by sudden drop in the population. It can be deciphered that 30% and 40% of wheat bran can be used as a potential liquid media formulation to
develop a low-cost liquid consortium biofertilizer on the condition of its optimization to enhance the growth and shelf life.
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Samredhi (2022). Assessment of low-cost media for production of liquid wheat consortium biofertilizer (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.