CHARACTERIZATION OF WILD VIGNA SPECIES FOR SEEDLING WATER-LOGGING TOLERANCE AS REVEALED BY PHYSIO-CHEMICAL AND EXPRESSION PROFILING
Kumar I, Sandeep
Banda University of Agriculture & Technology, Banda-210001, Uttar Pradesh, India
Mungbean is the most important short duration grain legume. It is consumed as food and feed, fodder and manure crop. It has wider adaptability to array of environmental conditions, shorter life cycle and improves the soil health through symbiosis. These features indicated that there is an ample scope of vertical and horizontal expansion of mungbean worldwide. However, it is much weather sensitive crop in which water logging at early crop growth stage caused sever plant loss and stunted growth. The development of water logging tolerant mungbean varieties required the potential donors. The crop wild relatives (CWRs) and cross species Vigna possessed improved tolerance can be identified and introgressed in mungbean. The 32 Vigna accessions including cultivated and wild belong to nine different species were evaluated and six genotypes namely PRR 2008-2, PRR 2008-2-1, TCR-93, L-24, TCR-20 and W-17 were found as water logging tolerant. These tolerant genotypes had better survival, strong root system, proper shoot elongation and delayed chlorophyll degradation. The two genotypes PRR 2008-2-1 and PRR 2008-2 showed induction of adventitious roots from the hypocotyl region under water logging stres. The histochemical staining indicated the lower cell death in leaves of PRR 2008-2. The PRR 2008-2 showed formation of aerenchyma as adoptive mechanism as compare to IPM 2-3. The higher activity of ADH, proline and O2 were was recorded in PRR 2008-2 whereas it was lower in IPM 2-3. The expression of candidate genes and transcriptional factors provides a fundamental resources for understanding signaling behaviour, hormonal regulation and antioxidant homeostatsis involved in water logging tolerance.
Sandeep Kumar. 2022. CHARACTERIZATION OF WILD VIGNA SPECIES FOR SEEDLING WATER-LOGGING TOLERANCE AS REVEALED BY PHYSIO-CHEMICAL AND EXPRESSION PROFILING. Thesis, M.Sc.