Biochemical markers for yellow mosaic virus resistance in soybean cultivar JS335 introgressed with resistant genes from wild/cultivated species
Loading...
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Punjab Agricultural University, Ludhiana
Abstract
Present investigation reports the biochemical parameters in yellow mosaic disease (YMD)
susceptible soybean genotype JS335, resistant genotypes G. soja (wild species) and G. max
Cv. SL525 and resistant versions of JS335 introgressed with genes from wild/cultivated
species. Leaves from different soybean genotypes were collected at 1, 10, 20 and 30 days post
YMD infection under field conditions. Viral infection resulted in the formation of chlorotic
yellow patches on leaf lamina and reduced leaf size of susceptible genotype JS335 whereas
G. soja, SL525 and resistant lines of JS335 with G. soja (JS-GS) and SL525 (JS-SL) did not
show disease symptoms. JS-GS and JS-SL lines showed higher seed yield and 100 seed
weight as compared to susceptible genotype JS335. The activities of antioxidant enzymes
[glutathione reductase (GR), glutathione-s-transferase (GST), peroxidase (POD), polyphenol
oxidase (PPO) and superoxide dismutase (SOD)], phenolic constituents and non-enzymatic
antioxidants (proline, glutathione, hydrogen peroxide, malondialdehyde and glycine- betaine)
increased significantly from day 1 to day 30 post YMD infection with lower mean contents in
leaves of JS-GS and JS-SL lines in comparison to diseased JS335 leaves at all the stages of
crop development. Higher contents of total soluble proteins, total soluble sugars, reducing
sugars and lipids were recorded in seeds of improved JS335 lines in comparison to
susceptible JS335 whereas amino acids, phenolic constituents, sucrose and minerals showed
the opposite trend. The resistant JS335 lines showed improved resistance to yellow mosaic
disease and higher physiological parameters indicating them to be better in performance as
compared to susceptible JS335 under local environment of Punjab.
Description
Keywords
null