A comparison of some serological tests for diagnosis of brucellosis in vaccinated and unvaccinated bovines
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Date
2018
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
Bovine brucellosis is an emerging zoonotic disease of livestock and is of major threat to dairy industry causing late term abortions and infertility. For the control and eradication of brucellosis, accurate serodiagnostic tests are required. The present study was carried out for serodiagnosis of brucellosis in vaccinated and unvaccinated bovines and to evaluate the Superagglutination test by comparing its efficacy with other serological tests like Rose Bengal Plate Test (RBPT), Microagglutination test (MAT), Indirect Haemagglutination Assay (IHA), Indirect Enzyme Linked Immunosorbent Assay (iELISA) based on sensitivity, specificity and predictive values. The seroprevalence of brucellosis from 486 animals by RBPT, MAT, IHA, i-ELISA and Superagglutination test was 224 (46.09%), 258 (53.09%), 216 (44.44%), 264 (54.32%) and 279(57.41%), respectively. The mean titre of MAT in unvaccinated animals was 1.84±0.91 and in vaccinated animals was 2.63±0.56. Superagglutination showed highest sensitivity (98.21%) when compared with RBPT and highest negative predictive value (NPV) of 98.07% when compared with RBPT and IHA. The specificity (85.53%) and positive predictive value (PPV) of 88.17% of Superagglutination was highest when compared with MAT. Superagglutination showed highest agreement with MAT. Agreement of Superagglutination with other test was found to be strong. Keeping i-ELISA as gold standard, the sensitivity of RBPT, MAT, IHA, c-ELISA and Superagglutination was 79.55%, 87.50%, 77.27%, 100.00% and 92.05%, while specificity was 93.69%, 87.84% and 87.84%, 66.67% and 83.78%, respectively. RBPT showed PPV of 93.75% and NPV of 79.39% while PPV of MAT and IHA was 89.53% and 88.31% while NPV 85.53% and 76.47%, respectively. C-ELISA showed PPV 97.37% and NPV 100.00%. Superagglutination showed PPV 87.10% and NPV 89.86%. All the tests showed strong degree of agreement while c-ELISA showed moderate degree of agreement when compared with i-ELISA. Superagglutination showed highest sensitivity and negative predictive value among all the tests with advantage of better visualization and ease of carrying of the test.
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