Studies on isolation and characterization of rhizosperic fungi for phosphate solubilisation activity under in vitro condition
Loading...
Date
2021
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),
Abstract
ABSTRACT
Name: DEEKSHA CHAUDHARY Id. No. : 4889
Semester and year of admission: 1 sem. 2019-2020 Degree: M. Tech/M.sc
Major: Biotechnology College: Biotechnology
Advisor: Dr. Akash Tomar
Thesis Title: “Studies on isolation and characterization of rhizospheric fungi for phospahte solubilisation activity under invitro condition.”
Plant require phosphorus, a vital plant nutrient, it is found in fixed forms in most soil. Thus, poor availability of P in soil and consequent P-deficiency represents a major constraint to crop production globally. This has led to a search for environment-friendly and economically feasible alternative strategies for improving crop production in low or P deficient soils, PSM are known to bring about mobilisation of insoluble phosphates and stimulate plant development even under the condition of phosphorus deficiency. Mineral solubilization is the mechanism of soil microorganisms releasing bound nutrients and making them available for plant uptake by one of three mechanisms: acidification of the medium, formation of chelating metabolites, or redox activity. The nutrient-solubilizing bacteria produce a halo zone around their colony, which was used to determine their solubilization capacity. The phosphate solubilizing fungus (PSF) secretes some kinds of organic acids which act on insoluble phosphates and convert the same into soluble form, thus providing phosphorous to plant.
Ninteen of the rhizospheric soil sample were collected from the different region of the district Meerut and Muzaffarnagar and were sceened on PDA and PVK plates to obtain variety of fungal isolates. 17 fungal cultures were isolated from the 19 soil samples and 03 isolates were obtained from different studies. These 20 isolates were screened and selected on the basis of its ability to form halo (zone of solubilisation) zone on PVK medium plates. Out of 20 isolates 15 isolates were found to solubilise the TCPs. Further, colony morphology and microscopic studies shows that the 12 isolates (S2,S3.A,S5,S7.AS8,S10,S12,SS6.A,SS6.B ISOL.1,S4 and S7.B) belong to the genera Aspergillus, 04 isolate (S11,S13,S3.B,S7.C) belongs to the Trichoderma, 03 Penicillium (S1,S15 and ISOL.2) and 01 of Fusarium spp.
15 isolates (Aspergillus and Penicillium) showed the zone for P solubilisation while the other 05 fungal isolates (Trichoderma 04 no and Fusarium 01 no) did not show any zone of solublization. However, Trichoderma isolates showed production of acid in PVK medium which was evident by appearance of red colour zone surrounding the culture colony. Among all of the Aspergillus isolates maximum solubilisation efficiency (SE) was shown by S2 of 2.62 followed by SS6.B(2.33), S8(2.25), S3.A(2.08) and S7.A(2.00), the minimum (SE) was shown by isolate S12 of 0.43. While considering about the SI value isolate SS6.A had the maximum value of 1.19. Among all three Penicillium isolates tested for P solubilisation, ISOL.2 was recorded to have more efficiency (SE) of 1.08 than other isolates followed by S1(1.00) and S15 (0.58) on the twelfth day of observation. If considering about the solubilisation index (SI) then, ISOL.2 shows maximum SI value of 1.13 followed by S1(1.10) and S15(1.05). Comparatively among all the Aspergillus spp. S3.A, S10 and S2 can be considered as the best solubilisers while S15 and ISOL.2 were better among the Penicillium species.
(Akash Tomar) (Deeksha Chaudhary)
ADVISOR AUTHOR