Anti-inflammatory Activity of Emu Oil in Indomethacin Induced Inflammatory Bowel Disease in Rats
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Date
2015-07
Journal Title
Journal ISSN
Volume Title
Publisher
Council of the National Academy of Sciences
Abstract
Inflammatory bowel disease is a chronic condition
affecting human and animal species. The present
study was taken up with the objective to test an animal
product, emu oil against inflammatory bowel disease and to
evaluate its activity in comparison to aloe vera (plant
product) alone and in combination with emu oil. Experiment
was performed in six groups containing 6 rats
each. Rats were pre-treated for five consecutive days with
sulfasalazine [100 mg/kg], aloe vera [400 mg/kg], emu oil
[10 ml/kg] and the combination of aloe vera and emu oil to
groups III, IV, V and VI respectively. The condition was
induced using two consecutive subcutaneous injection of
indomethacin [10 mg/kg] on day 4 and 5 in all groups
except group I (control). Rats were sacrificed on day 8 and
samples were collected for further analysis. Macroscopic
lesions include ulceration [pin point to linear], perforation
and necrotic epithelium in caecum. Microscopically, there
was ulceration [loss of epithelium] and increased cellular
infiltration. Emu oil was analysed through Gas chromatography–
Mass spectrometry for fatty acid profiling and
was found to contain mixture of unsaturated and saturated
fatty acids. This was evident through the analysis of
inflammatory and oxidative stress parameters of group V
when compared to groups III and IV. However, the combination
of aloe vera and emu oil showed better activity
than all other groups. Hence it was concluded that emu oil
might be attempted to treat the inflammatory conditions of
gastro-intestinal tract as a part of alternative medicine.
Description
TNV_PNAS_2015_85(3)831-837
Keywords
Veterinary Science