Nano-zinc oxide (ZnO)-polymer seed coatings and their effect on soil microbes in maize (Zea mays L.)
| dc.contributor.advisor | Kalia, Anu | |
| dc.contributor.author | Tondey, Manisha | |
| dc.date.accessioned | 2021-03-03T07:09:01Z | |
| dc.date.available | 2021-03-03T07:09:01Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | The present work aimed for the synthesis of ZnONPs from zinc acetate or zinc chloride as precursor salts by using various reducing agents such as natural polymers (starch, cellulose, potato-extract), bovine serum albumin, and thiourea. On UV-Vis absorption spectroscopy analysis, the prepared ZnONPs exhibited characteristic absorption peaks ranging from 210 to 360 nm wavelength. The FT-IR spectroscopy of the synthesized ZnONPs depicted presence of Zn metal-oxide functional groups. Transmission electron microscopy analysis revealed quasispherical shapes of ZnONPs with an average particle size of <100 nm. The synthesized ZnONPs were also evaluated for their antimicrobial potential for plant beneficial and phyto-pathogenic bacterial and fungal cultures. Wet-chemistry derived ZnONPs (at 40 mg L-1) exhibited antimicrobial activity against Dickeya sp. while at 20 mg L-1 concentration radial mycelial growth of Macrophomina phaseolina was inhibited. Apart from antimicrobial potential, the free radical scavenging or antioxidant activity of prepared ZnONPs as determined through DPPH radical scavenging method. It was observed to be vary considerably among the ZnONPs synthesized using different reducing agents and hence is anticipated to positively affect seed germination. The effect of application of ZnONPs as seed priming and coating treatment on germination and seedling growth traits was evaluated for two maize varieties PMH-1 and J-1006 using different concentrations (0, 5, 10, 20, 40 mg L-1) of ZnO NPs and bulk ZnSO4 suspensions under laboratory conditions. Seed coating with 20 mg L-1 of ZnONPs exhibited significantly higher percent germination, root and shoot length, and fresh and dry weight of root besides number of lateral roots of the maize seedlings (variety J-1006) as compared to bulk ZnSO4 treatments. Further, ZnONPs priming and coating treatments were also evaluated for enhancing vegetative growth and yield attributing parameters under field conditions during spring and kharif seasons in the year of 2017-18 and 2018-19. The application of ZnONPs (at 40 mg L-1 concentration) significantly enhanced the total chlorophyll content, available soil nutrient content (Nitrogen and Phosphorus), improved the total soil microbial counts and soil enzyme activities(dehydrogenase, and acid and alkaline phosphatase enzyme activities). Whereas, a significant increase in the available soil potassium, and zinc contents and numerical increase in vegetative and yield parameters (number of plants, plant height, stover yield, plant biomass) was recorded on treatment of seeds with ZnONPs at 20 mg L-1 in maize variety J-1006 as compared to bulk ZnSO4 and control treatments. These findings suggest positive effect on growth and yield attributing characters in maize on seed priming and coating with ZnONPs. Therefore, use of ZnONPs at low concentrations as seed priming/ coating agent can effectively improve productivity and soil health. | en_US |
| dc.identifier.citation | Tondey, Manisha (2020). Nano-zinc oxide (ZnO)-polymer seed coatings and their effect on soil microbes in maize (Zea mays L.) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810161899 | |
| dc.keywords | Maize, ZnO nanoparticles, antimicrobial activity, priming and coating, seed germination, yield | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 107 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Nano-zinc oxide (ZnO)-polymer seed coatings and their effect on soil microbes in maize (Zea mays L.) | en_US |
| dc.sub | Microbiology | en_US |
| dc.theme | Nano-zinc oxide (ZnO)-polymer seed coatings and their effect on soil microbes in maize (Zea mays L.) | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Nano-zinc oxide (ZnO)-polymer seed coatings and their effect on soil microbes in maize (Zea mays L.) | en_US |
| dc.type | Thesis | en_US |