STUDIES ON DEVELOPMENT OF PEACH GENIC-SSRs AND TRANSFERABILITY TO SOME ROSACEAE SPECIES
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Date
2012
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Abstract
ABSTRACT
In the present investigation EST – SSR markers were developed for peach and were
further used for cross-transferability studies in some Rosaceae species viz., apricot, apple,
rose and strawberry. EST sequences of Prunus persica available on NCBI website
(www.ncbi.nlm.nih.gov/nucest) were screened for SSR motifs using an online tool, SSRIT
(www.gramene.org/db/searches/SSRtool). 240 EST sequences were detected to contain SSRs,
out of which 52 EST-SSRs were designed using PRIMER 3 software (www.frodo.wimit.
edu/primer3/). Out of 52 EST-SSRs, 43 were custom synthesized. In the wet lab
experimentation , DNA from fresh, young leaves of six genotypes of peach, four genotypes
each of apricot, apple, rose and strawberry was isolated by CTAB method (Doyle and Doyle,
1987) for polymorphism and transferability studies. These 43 custom synthesized primers
were used for polymorphism study in peach. Out of 43 primers, 38 primers showed
amplification, out of which 20 were polymorphic, revealing 68% polymorphism in peach.
These 20 polymorphic primers of peach were then further used to carry out transferability
studies in apricot, apple, rose and strawberry. 50%, 95%, 95% and 45% transferability was
observed in apricot, apple, rose and strawberry, respectively. Similarity matrices and
dendrograms were generated using NTSys ver.2.02h and dissimilarity matrices and rooted
trees were generated using DARwin 5 ver.5.0.155 for polymorphism data of all genotypes
under study. All the 22 genotypes under study were grouped into two main clusters. Rooted
tree of 22 genotypes revealed that ‘Lazy’ cultivar of apricot was singled out from rest of the
cluster. Thus it is concluded from the present study that ESTs of peach produced high
polymorphism in different Rosaceous species indicating their cross-transferability and use
across different members of Rosaceous family.
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Keywords
---, EST – SSR markers, peach ,Rosaceae species