STUDIES ON ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI FROM SHEEP, GOATS AND THEIR HANDLERS
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Date
2016
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Division of Veterinary Public Health and Epidemiology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
Abstract
A total of 170 samples comprising of 30 faecal samples and 30 rectal swabs of each sheep and goats, 25 stool samples and 25 hand swabs of their handlers were collected from in and around Jammu district and processed for the isolation of E. coli. Sixty five biochemically confirmed isolates including 27 of sheep, 26 of goats and 12 of their handlers were obtained. In vitro virulence characterization of isolates exhibited that 55.5% of isolates of sheep and 50% isolates of goats took colour of Congo red dye, similarly 25.9% and 34.6% isolates of sheep and goats respectively were haemolytic on blood agar, but none of human isolate was found pathogenic on both of the in vitro pathogenicity assays. Target specific primers of mPCR for stx1, stx2, eaeA and hlyA detected STEC in 28 isolates, with prevalence rate of 55.5%, 50%, and 0% in sheep, goats and their nomadic handlers respectively. All the isolates of handlers were negative for all genes and none of the sheep and goat isolate was found positive for eaeA gene. The antibiogram screening showed differential susceptibility pattern among E. coli isolates. Norfloxacin was found most potent drug with 84.6% sensitivity followed by Chloramphenicol (80%), Co-trimoxazole (75.3%) and Nalidixic acid (70.7%). However 60% of E. coli isolates were resistant to Ampicillin and Amikacin.