MOLECULAR CHARACTERIZATION OF BOVINE HERPESVIRUS-2 ISOLATED FROM THE CASES OF PSEUDO LUMPY SKIN DISEASE
Loading...
Date
2016-10-05
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
PVNR TVU
Abstract
The present study was undertaken to isolate Bovine herpesvirus type 2 (BHV-2) from suspected case of pseudo lumpy skin disease (PLSD), and its molecular characterization.
BHV-2 was isolated from sample by passaging 3 times in primary cells (lamb testicular and kidney cells), and MDBK cells then CPE was studied. Purification of BHV-2 from infected cell culture fluid was done using gradient ultracentrifugation. Transmission electron microscopy of purified virus showed hexagonal virus particles of size 200-250 nm diameter which approximated presence of BHV-2.
PCR product of partial gB gene from PLSD sample was sequenced and
16
analyzed. The sequence was found to have high identities with other BHV-2 sequences available with NCBI-GenBank. This was also reflected in the sequence identity matrices generated with BioEdit software. Multiple sequence alignment revealed highly conserved regions in the target sequence.
Phylogenetic tree reconstruction showed available BHV-2 isolates clustering together. BHV-2 sequence obtained from isolate clustered with BHV-2 sequences from Germany and Brazil isolates. All other human herpes viruses are clustered together, separately from BHV-2 sequences.
The results reveal the identity of the local virus isolate as BHV-2. It also confirms the presence of BHV-2 in India and warrants further studies on higher scale.
Description
Keywords
livestock, mastitis, animal husbandry, animal products, bacteria, biological phenomena, sampling, antibiotics, economic systems, diseases