PATHOGENICITY STUDIES OF BLUETONGUE VIRUS SEROTYPE 4 IN SHEEP

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Date
42674
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PVNR TVU
Abstract
In the present study an attempt was made to study the pathogenicity of Bluetongue virus (BTV) serotype 4 for 30 days followed by superinfection with BTV serotype 16 for next 30 days in 5 BTV seronegative Deccani sheep of 6-8 months age using the KC cell line (Culicoides sonorensis) adapted live virus. The effect of virus was studied by inoculating the live virus and by observing the clinical symptoms, hematological parameters such as RBC count, WBC count, haemoglobin percentage and BTV antibody response by cELISA, SNT and finally by virus isolation from the blood of experimentally infected animals. Serotypes 4 and 16 were propagated in KC cell line and titrated in BHK- 21 (Baby Hamster Kidney cells) cells. The live virus was inoculated in such a way that each of the 4 animals received a dose of 106.0 TCID50 live virus adapted in KC cell culture and 1 control animal received uninfected KC cell suspension. Infected sheep showed mild clinical signs of BT in BTV-4 infection where as the clinical signs were not observed after BTV-16 super infection. Sheep no 239 and 231 showed mouth lesions (clinical signs) 8 and 10 dpi (days post infection). Rise in temperature ranging from 103.7-105.4o F was observed in BTV- 4 infection and a range of 103.3-105o F in BTV-16 superinfection. All the infected sheep seroconverted between 5 and 7 dpi as detected by c-ELISA. Neutralizing antibodies were detected 7 dpi in BTV-4 infection and 15 dpi in BTV-16 superinfection by SNT. Infected sheep did not show change in WBC count, RBC count and hemoglobin percentage when compared to the control animal. Finally, BTV-4 could be isolated from blood of infected animals. Our current study aided in developing a reproducible experimental model of BT in sheep that can be used to better characterize the different clinical signs of the disease, pathogenicity and infection kinetics.
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biological phenomena, livestock, diseases, viruses, sampling, elisa, application methods, pcr, electrophoresis, productivity
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