POLYMORPHISM IN BMP-15 GENE IN MARWARI GOAT
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Date
22-04-10
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Rajasthan University of Veterinary and Animal Sciences Bikaner-334001 (Rajasthan)
Abstract
The present study was undertaken to investigate polymorphism in BMP-15 (Fec XB and Fec XG in exon-2) gene in Marwari goats by RFLP
pattern. Samples were collected from 60 unrelated goats of Marwari breed
maintained at Daiya, kodemdaser, Deshnok and kalyansar farm of Bikaner
district under the goat scheme. Blood samples were collected from Jugular
vein of Marwari goats. Genomic DNA was isolated from collected samples by
phenol chloroform extraction method. Amplification of BMP- 15 gene was
done by Polymerase Chain reaction using corresponding primers having product size of 153 bp and 141 bp for Fec XB and Fec XG gene respectively.
DNA isolated from samples revealed proper amplification of exon-2 BMP-15 gene at Fec XB and Fec XG loci. Later on amplicons were purified by phenol
extraction method. Restriction digestion of purified amplicons was done using
appropriate restriction endonuclease enzyme for studying Restriction
fragment length polymorphism in amplified PCR product. All amplified
products revealed monomorphism after restriction digestion possessed similar band pattern for exon-2 BMP-15 at Fec XB and Fec XG loci.
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Keywords
byproducts, diseases, biological phenomena, livestock, infiltration, animal tissues, proteins, research methods, sampling, dna