Skip navigation
Beta Version

Thesis

STUDIES ON DIVERSE PLANT GROWTH PROMOTING RHIZOBACTERIA ASSOCIATED WITH RHIZOSPHERE OF KIWI VINES

en
7
0

Attachments [ 1 ]

Final PDF Malvika 2015.pdf(3.65 MB)

(Basic Science(M.Sc))

More Details

2015
SHIRKOT, C.K.
M.Sc

ABSTRACT Plant hormones are chemical messengers that affect a plant's ability to respond to its environment. Hormones are organic compounds that are effective at very low concentration; they are usually synthesized in one part of the plant and are transported to another location. Botanists recognize five major groups of hormones: auxins, gibberellins, ethylene, cytokinins, and abscisic acid. IAA (indole-3-acetic acid) is the member of the group of phytohormones and is generally considered the most important native Auxin. It functions as an important signal molecule in the regulation of plant development including organogenesis, tropic responses, cellular responses such as cell expansion, division, differentiation, and gene regulation. Therefore, the objective of the present study was to isolate and screen IAA producing rhizobacteria from kiwi vines and to optimize cultural conditions for maximum production of IAA. One hundred and fifty seven isolates (sixty three rhizospheric and ninety four root endophytic isolates were isolated from different cultivars of kiwi vines and were screened for IAA production. These IAA producing isolates were further screened for P-solubilization on PVK media, siderophore production on CAS medium and their ability to grow on nirtrogen free medium. Overall maximum IAA producing twenty isolates were selected for screening of other PGP traits. Variation was also observed in their plant growth promoting (PGP) activities such as IAA production, P-solubilization and siderophore production. Out of twenty, majority isolates exhibited antifungal activity and showed enzyme production. Overall six most efficient bacterial isolates were finally selected and evaluated for plant growth promotion of tomato and kiwi seedlings under net house conditions. The most efficient isolate B2S10 was further subjected to optimization of cultural conditions for IAA production. Maximum IAA production of (55μg/ml) was observed after 96 hours of incubation at 35⁰C temperature, pH 9 in LB broth amended with 1.00 (%) tryptohan. The most efficient isolate was identified as Serratia marcescens by 16SrRNAgene sequence analysis

Comments

(Leave your comments here about this item.)

Item Analytics

Item Views and Downloads

Geographical spread of Visitors

Country City Visitors