Cloning and characterization of water stress inducible APETALA type 2 transcription factor gene from rice (Oryza sativa)
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Date
2009
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Publisher
iari, DIVISION OF BIOCHEMISTRY
Abstract
Drought stress is one of the major abiotic stresses limiting plant growth and
productivity. During the process of plant adaptation to drought stress, plants have
developed sophisticated systems to tolerate drought which involves physiological,
biochemical and molecular responses.Transcriptome analysis using microarray
technique has proven very useful for discovering stress inducible genes. Among these
are stress inducible regulatory genes that encode proteins such as transcription factors.
In the present study, the cDNA of gene SIAP2/ERF N22 encoding a transcription factor
of AP2/ERF family has been isolated and characterized from Oryza sativa L.cv.N22 .
The cDNA sequence of SIAP2/ERF N22 gene (732bp) was amplified using
gene specific AP2/ERF primers. The amplicon was cloned and sequenced. In the
nucleotide sequence alignment analysis, cDNA showed perfect match with coding
sequence of SIAP2/ERF N22 gene.The protein sequence alignment showed that the
SIAP2/ERF N22 cDNA encodes protein of 243 amino acids. The phylogenetic analysis
of AP/ERF family revealed that SIAP2/ERF N22 belongs to SHN clade of AP2/ERF
proteins which activates wax biosynthesis in Arabidopsis. Hence a comparison of
epicuticular wax content was done at varied relative water content levels in leaves of
Oryza sativa L.cv N22. A higher epicuticular wax content was observed at decreased
RWC. Further RT-PCR analysis was done under control and water deficit condition. A
faint band was observed in control where as drought stressed sample showed intense
band. These results showed that the SIAP2/ERF N22 gene is inducible in drought.
Further,the expression can be positively correlated with wax content.
Description
T-8124